Romero N M, Parro V, Malpartida F, Mellado R P
Centro Nacional de Biotecnología, Facultad de Ciencias UAM, Madrid, Spain.
Nucleic Acids Res. 1992 Jun 11;20(11):2767-72. doi: 10.1093/nar/20.11.2767.
A DNA fragment of Streptomyces fradiae is able to activate the antibiotic actinorhodin biosynthetic pathway when cloned in Streptomyces lividans. The activator DNA region has been sequenced and its transcription initiation and termination sites accurately mapped in vivo. This DNA encodes a 132 nucleotides long transcript which is apparently responsible for the actinorhodin production phenotype, possibly acting as an antisense RNA. The sequence of the activator gene revealed no homology with any other known Streptomyces coelicolor genes concerned with actinorhodin biosynthesis or its pleiotropic regulation.
弗氏链霉菌的一个DNA片段克隆到天蓝色链霉菌中时，能够激活抗生素放线紫红素的生物合成途径。已对激活剂DNA区域进行了测序，并在体内精确绘制了其转录起始和终止位点。该DNA编码一个132个核苷酸长的转录本，它显然与放线紫红素的产生表型有关，可能作为反义RNA发挥作用。激活剂基因的序列与其他任何已知的与放线紫红素生物合成或其多效性调控有关的天蓝色链霉菌基因均无同源性。
