角鲨烯对环磷酰胺诱导毒性的影响。

Effect of squalene on cyclophosphamide-induced toxicity.

作者信息

Senthilkumar Subramanian, Devaki Thiruvengadam, Manohar Bhakthavatchalam Murali, Babu Munismay Suresh

机构信息

Department of Biochemistry, University of Madras, Guindy campus, Chennai, Tamilnadu 600025, India.

出版信息

Clin Chim Acta. 2006 Feb;364(1-2):335-42. doi: 10.1016/j.cca.2005.07.032. Epub 2005 Sep 8.

DOI:10.1016/j.cca.2005.07.032

PMID:16150433

Abstract

BACKGROUND

Toxicity due to drugs used for neoplastic disorders is extensively documented. Cyclophosphamide (CYP) is a widely used antineoplastic drug, which could cause toxicity of normal cells due to its toxic metabolites. We evaluated the protective role of squalene (SQ) in the toxicity induced by cyclophosphamide.

METHODS

The activities of serum marker enzymes, clinical chemistry parameters and histopathology studies were done according to the standard procedures in the control and experimental groups of rats.

RESULTS

Toxicity of the organs like heart, kidney and liver was evidenced from significant (P<0.05) increases of CK, LDH, AST, ALT, ALP, urea, creatinine and total bilirubin in cyclophosphamide- (150 mg/kg for 2 days) administered rats. Abnormal activities of these enzymes in the organs and serum total protein and cholesterol were also observed. No significant changes were observed in triglycerides in serum. Squalene oral treatment exerted protection towards these organs at a dose of 0.4 ml/day/rat. Histopathological examinations also confirmed the protective efficacy of squalene.

CONCLUSION

Squalene may be efficacious as a cytoprotectant in cyclophosphamide-induced toxicities.

摘要

背景

用于肿瘤疾病的药物毒性已有广泛记载。环磷酰胺（CYP）是一种广泛使用的抗肿瘤药物，其毒性代谢产物可导致正常细胞毒性。我们评估了角鲨烯（SQ）对环磷酰胺诱导的毒性的保护作用。

方法

按照标准程序对大鼠对照组和实验组进行血清标志物酶活性、临床化学参数及组织病理学研究。

结果

给予环磷酰胺（150mg/kg，连续2天）的大鼠，其心脏、肾脏和肝脏等器官的毒性表现为肌酸激酶（CK）、乳酸脱氢酶（LDH）、天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、碱性磷酸酶（ALP）、尿素、肌酐和总胆红素显著升高（P<0.05）。还观察到这些酶在器官中的异常活性以及血清总蛋白和胆固醇的变化。血清甘油三酯未观察到显著变化。角鲨烯经口给药，剂量为0.4ml/天/大鼠，对这些器官具有保护作用。组织病理学检查也证实了角鲨烯的保护效果。