Hangalapura Basavarajappa N, Kaiser Michael G, Poel Jan J van der, Parmentier Henk K, Lamont Susan J
Adaptation Physiology Group, Wageningen Institute of Animal Sciences, Wageningen University, 6709 PG, Wageningen, The Netherlands.
Dev Comp Immunol. 2006;30(5):503-11. doi: 10.1016/j.dci.2005.07.001. Epub 2005 Aug 1.
The effects of cold stress, immunization and genetic selection on the expression of mRNA for cytokine genes in poultry have not been completely elucidated. Therefore, in the present experiment, using real-time quantitative RT-PCR, we evaluated the effect of cold stress and immunization with complete Freund's adjuvant (CFA) on expression of mRNA for pro-inflammatory (interleukin-1beta [IL-1beta], IL-6, IL-12beta), Th(1) (IFN-gamma and IL-2), and Th(2) (IL-4 and IL-10) cytokine genes in peripheral blood leukocytes (PBL) of chicken lines divergently selected for either high or low antibody responses. Irrespective of the duration, cold stress enhanced expression of mRNA for IL-1beta, IL-6, IL-12beta and IL-4 cytokine genes in both selection lines. These results indicate that cold stress stimulates both the innate and parts of the adaptive cellular immune system. Immunization with CFA resulted in higher expression of mRNA for pro-inflammatory cytokines and lower expression of mRNA for both Th(1) and Th(2) cytokines.
冷应激、免疫接种和基因选择对家禽细胞因子基因mRNA表达的影响尚未完全阐明。因此，在本实验中，我们使用实时定量逆转录聚合酶链反应（RT-PCR），评估了冷应激和用完全弗氏佐剂（CFA）免疫接种对高抗体反应或低抗体反应差异选择鸡系外周血白细胞（PBL）中促炎（白细胞介素-1β[IL-1β]、IL-6、IL-12β）、Th(1)（干扰素-γ和IL-2）和Th(2)（IL-4和IL-10）细胞因子基因mRNA表达的影响。无论持续时间如何，冷应激均增强了两个选择系中IL-1β、IL-6、IL-12β和IL-4细胞因子基因的mRNA表达。这些结果表明，冷应激刺激了先天性和部分适应性细胞免疫系统。用CFA免疫接种导致促炎细胞因子的mRNA表达较高，而Th(1)和Th(2)细胞因子的mRNA表达较低。
