Pierzchala P A, Dorn C P, Zimmerman M
Biochem J. 1979 Dec 1;183(3):555-9. doi: 10.1042/bj1830555.
A new fluorogenic peptide substrate for plasmin, 7-(N-succinoylalanylphenylalanyl-lysylamido)-4-methylcoumarin trifluoroacetate salt, was prepared that can be used in a simple and direct assay. The results obtained by the assay method are linear over a wide range of enzyme concentrations and sensitive enough to detect as little as 10(-5) CTA units of plasmin. By making use of the inhibitor Trasylol and the differences in kinetic constants, plasmin can be specifically assayed even in the presence of the plasminogen activator thrombin, as well as in culture fluids from HeLa cells.
制备了一种用于纤溶酶的新型荧光肽底物，即7-（N-琥珀酰丙氨酰苯丙氨酰赖氨酰胺）-4-甲基香豆素三氟乙酸盐，它可用于一种简单直接的检测方法。通过该检测方法获得的结果在很宽的酶浓度范围内呈线性，并且灵敏度足以检测低至10^(-5) CTA单位的纤溶酶。利用抑制剂抑肽酶以及动力学常数的差异，即使在存在纤溶酶原激活剂凝血酶以及来自HeLa细胞的培养液的情况下，也能够特异性地检测纤溶酶。
