Han Guangye, Li Jun, Chen Guiying, Ling Lin, Li Shuqin, Khorobrykh Andrew A, Zharmukhamedov Sergei K, Klimov Vyacheslav V, Kuang Tingyun
Photosynthesis Research Center, Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, The Chinese Academy of Sciences, Beijing 100093, China.
J Photochem Photobiol B. 2005 Nov 1;81(2):114-20. doi: 10.1016/j.jphotobiol.2005.04.009. Epub 2005 Sep 9.
The efficiency of a trinuclear and two binuclear manganese complexes in reconstituting electron transport and O(2) evolution activity in Mn-depleted Photosystem II preparations is analyzed. The trinuclear Mn-complex is more efficient than two binuclear Mn-complexes in restoring oxygen evolution, but it is less effective as an electron donor than binuclear Mn-complexes. It is inferred from our results that recovery of electron transport and O(2) evolution with polynuclear Mn-complexes is affected with different factors. Moreover, the trinuclear Mn-complex is extremely sensitive to the addition of CaCl(2). It is suggested that there is an interaction between Ca(2+) and carboxyl within the trinuclear Mn-complex during photoactivation and this interaction benefits the ligation of Mn atom to the apo-WOC and form an active WOC. Binuclear Mn(III)Mn(III) complex shows slightly higher efficiency than binuclear Mn(III)Mn(IV) complex in restoration of O(2) evolution activity. The efficiency of three Mn-complexes in the reconstitution of WOC is in an order: trinuclear Mn(3)(III)>binuclear Mn(III)Mn(III)>binuclear Mn(III)Mn(IV).
分析了一种三核和两种双核锰配合物在重建缺锰光系统II制剂中的电子传递和O₂ 释放活性方面的效率。三核锰配合物在恢复氧气释放方面比两种双核锰配合物更有效，但作为电子供体，其效果不如双核锰配合物。从我们的结果推断，多核锰配合物对电子传递和O₂ 释放的恢复受不同因素影响。此外，三核锰配合物对添加CaCl₂ 极为敏感。表明在光激活过程中，Ca²⁺ 与三核锰配合物中的羧基之间存在相互作用，这种相互作用有利于锰原子与脱辅基水氧化复合物（apo-WOC）连接并形成活性水氧化复合物（WOC）。双核Mn(III)Mn(III)配合物在恢复O₂ 释放活性方面比双核Mn(III)Mn(IV)配合物略高。三种锰配合物在重建WOC方面的效率顺序为：三核Mn₃(III)>双核Mn(III)Mn(III)>双核Mn(III)Mn(IV)。
