细胞角蛋白13基因在口腔鳞状细胞癌中的表达及意义
[Expression and significance of cytokeratin 13 gene in oral squamous cell carcinoma].
作者信息
Lv Xiao-zhi, Chen Wan-tao, Zhang Chen-ping
机构信息
Department of Oral and Maxillofacial Surgery, Ninth People's Hospital, School of Stomatology, Shanghai Second Medical University, Shanghai 200011, China.
出版信息
Shanghai Kou Qiang Yi Xue. 2005 Aug;14(4):378-81.
PURPOSE
To investigate the expression and its significance of cytokeratin 13 gene in oral squamous cell carcinoma (OSCC).
METHODS
Differential CK13 gene expression between 30 cases of paired normal and cancerous tissues were detected by reverse transcription-PCR (RT-PCR), and immunohistochemistry was conducted using monoclonal antibody against CK13 in them. The data were analyzed with SAS6.12 software for Fisher's exact test.
RESULTS
RT-PCR results demonstrated that CK13 gene mRNA was upregulated in 27 cases of normal tissues than in paired cancerous samples, by 4.2-fold in total; and immunostain results revealed that expression of CK13 protein between normal oral squamous cell and OSCC, high differentiated OSCC and poor differentiated ones were significantly decreased (P<0.05).
CONCLUSIONS
CK13 gene might play an important role in OSCC carcinogenesis and be regulated at post-transcriptional levels. Further research will contribute to confirm its role and mechanism.
目的
探讨细胞角蛋白13基因在口腔鳞状细胞癌（OSCC）中的表达及其意义。
方法
采用逆转录-聚合酶链反应（RT-PCR）检测30例配对的正常组织和癌组织中细胞角蛋白13基因的差异表达，并用抗细胞角蛋白13单克隆抗体对其进行免疫组织化学检测。采用SAS6.12软件进行Fisher确切概率法分析数据。
结果
RT-PCR结果显示，27例正常组织中细胞角蛋白13基因mRNA表达上调，与其配对的癌组织相比，总体上调4.2倍；免疫染色结果显示，正常口腔鳞状上皮与口腔鳞状细胞癌、高分化口腔鳞状细胞癌与低分化口腔鳞状细胞癌之间细胞角蛋白13蛋白表达均显著降低（P<0.05）。
结论
细胞角蛋白13基因可能在口腔鳞状细胞癌发生中起重要作用，且在转录后水平受到调控。进一步研究将有助于明确其作用及机制。
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