Farrukh Sanniya, Syed Serajuddaula, Pervez Shahid
Department of Pathology, Ziauddin University, Karachi, Pakistan E-mail :
Asian Pac J Cancer Prev. 2015;16(13):5489-92. doi: 10.7314/apjcp.2015.16.13.5489.
Gradual loss of cytokeratin 13 (CK13) may be linked with the severity of dysplastic changes and transformation to malignancy. In this study we assessed the differential expression of CK13 in normal, hyperplastic, dysplastic and cancerous oral mucosa.
A total of 93 oral biopsies were collected during the 2011-2014 period. The biopsies were characterized as normal (19), hyperplastic (21), severely dysplastic/carcinoma in situ (16) and invasive oral squamous cell carcinoma (OSCC) (37) after morphological assessment. Formalin fixed paraffin embedded sections were stained with a monoclonal antibody against CK13 using the Envision technique. Immunohistochemically stained slides were then analyzed for CK13 expression.
CK13 was consistently and diffusely expressed in all normal and hyperplastic tissue biopsies from oral mucosa. Severely dysplastic/carcinoma in situ biopsies showed complete loss in 50% of cases, while in the remaining 50% expression was very focal and weak. OSCC cases showed complete or near complete loss of CK13 in all cases. Few cases showed weak expression in keratin pearls only.
This study validates the utility of CK13 IHC as a useful immunohistochemical marker in routine diagnostic practice to make distinction between non-neoplastic from dysplastic and neoplastic (malignant) oral lesions.
细胞角蛋白13（CK13）的逐渐丧失可能与发育异常变化的严重程度以及向恶性转化有关。在本研究中，我们评估了CK13在正常、增生、发育异常和癌性口腔黏膜中的差异表达。
在2011年至2014年期间共收集了93份口腔活检标本。经形态学评估后，这些活检标本被分类为正常（19份）、增生（21份）、重度发育异常/原位癌（16份）和浸润性口腔鳞状细胞癌（OSCC）（37份）。采用Envision技术，用抗CK13单克隆抗体对福尔马林固定石蜡包埋切片进行染色。然后对免疫组织化学染色的玻片进行CK13表达分析。
CK13在所有正常和增生的口腔黏膜组织活检标本中持续且弥漫性表达。重度发育异常/原位癌活检标本中，50%的病例显示完全缺失，而其余50%的病例表达非常局限且微弱。OSCC病例在所有情况下均显示CK13完全或近乎完全缺失。少数病例仅在角化珠中显示微弱表达。
本研究验证了CK13免疫组化在常规诊断实践中作为一种有用免疫组化标志物的效用，可用于区分非肿瘤性、发育异常性和肿瘤性（恶性）口腔病变。
