Yang Jian, Yi Dinghua, Liu Jincheng, Liu Jian, Cao Ruijun, Qin Feng
Center of Cardiovascular Surgery, Xijing Hospital, The Fourth Military Medical University, Xi'an 710033, China.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2005 Aug;22(4):739-41.
In this study, heparin ionically coated polyvinyl chloride (PVC) material was prepared by heparin-benzalkonium chloride complex (Group A), heparin-benzalkonium bromide complex (Group B) and heparin-polyethyleneimine compound (Group C). Cytotoxicity evaluation was conducted by direct cell contact evaluation and MTT colorimetry method. The results showed Group A and Group B caused L-929 cells to die out while Group C showed good compatibility with cells. The OD levels of Group A and B were lower than that of the Group C in MTT test. Method A and method B of heparin coating had remarkable cytotoxicity, while method C had little cytotoxicity and could be further studied for clinical use.
在本研究中，通过肝素-苯扎氯铵复合物（A组）、肝素-苯扎溴铵复合物（B组）和肝素-聚乙烯亚胺化合物（C组）制备了肝素离子涂层聚氯乙烯（PVC）材料。通过直接细胞接触评估和MTT比色法进行细胞毒性评估。结果显示，A组和B组导致L-929细胞死亡，而C组与细胞表现出良好的相容性。在MTT试验中，A组和B组的OD水平低于C组。肝素涂层的方法A和方法B具有显著的细胞毒性，而方法C的细胞毒性较小，可进一步进行临床研究。
