LaFayette Nathan G, Skrzypchak Amy M, Merz Scott, Bartlett Robert H, Annich Gail M
Department of General Surgery, University of Michigan, Ann Arbor, Michigan, USA.
ASAIO J. 2007 Mar-Apr;53(2):159-62. doi: 10.1097/MAT.0b013e318031121f.
The development of a nonthrombogenic artificial surface for use with indwelling sensors or catheters remains an elusive goal despite decades of ongoing research. In vivo studies are both labor intensive and costly, and are therefore an inefficient way to rapidly screen possible surface materials. The following in vitro model used glass, polyvinyl chloride (PVC), and polypropylene test tubes incubated with 111In-labeled rabbit platelets and illustrated that, despite equivalent platelet count and function, platelet adhesion was greatest on glass (n = 13), with PVC (n = 17) at 67 +/- 8% and polypropylene (n = 13) at 43 +/- 5% when compared with glass. Extrapolating this method by coating test tubes with new, nonthrombogenic materials is a quick and reliable way to screen material before embarking upon more lengthy in vivo animal studies.
尽管经过数十年的持续研究，但开发一种用于植入式传感器或导管的非血栓形成人工表面仍然是一个难以实现的目标。体内研究既耗费人力又成本高昂，因此是快速筛选可能的表面材料的低效方法。以下体外模型使用玻璃、聚氯乙烯（PVC）和聚丙烯试管与111In标记的兔血小板一起孵育，结果表明，尽管血小板计数和功能相当，但与玻璃相比，血小板在玻璃上的粘附力最大（n = 13），PVC（n = 17）上的粘附力为67±8%，聚丙烯（n = 13）上的粘附力为43±5%。通过用新的非血栓形成材料涂覆试管来推断这种方法，是在开展更长期的体内动物研究之前筛选材料的快速且可靠的方法。
