Luo Peng, Liu Weiyong, Yang Chun, Zhou Hua, Cao Ruijun, Yang Jian
Department of Cardiovascular Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710033, China.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2011 Feb;28(1):90-4.
Based on non-enzymatic protein glycated reaction, the sodium periodate-oxidated low molecular weight heparin-antithrombin covalent complex (SPLMWATH) was produced. By using polyethyleneimine-glutaraldehyde bonding technique, polyvinyl chloride (PVC) tubings were coated with SPLMWATH, heparin and low molecular weight heparin (LMWH). Spectrophotometry and dynamic clotting time experiment were used to determine the synthetic ratio of SPLMWATH, graft density, coating leaching ratio and to evaluate the antithrombogenicity of different coating on the PVC tubings. The results showed that the synthetic ratio of SPLMWATH was approximately 55%, and compared with heparin coating and LMWH coating, the graft density of SPLMWATH coating on the PVC tubing was smaller, but its coating stability and antithrombogenicity were significantly better than that of heparin coating and LMWH coating on the PVC tubings.
基于非酶促蛋白质糖基化反应，制备了高碘酸钠氧化的低分子质量肝素-抗凝血酶共价复合物（SPLMWATH）。采用聚乙烯亚胺-戊二醛键合技术，将SPLMWATH、肝素和低分子质量肝素（LMWH）涂覆于聚氯乙烯（PVC）管材上。运用分光光度法和动态凝血时间实验测定SPLMWATH的合成比例、接枝密度、涂层浸出率，并评估不同涂层对PVC管材的抗血栓形成性能。结果表明，SPLMWATH的合成比例约为55%，与肝素涂层和LMWH涂层相比，SPLMWATH在PVC管材上的接枝密度较小，但其涂层稳定性和抗血栓形成性能明显优于PVC管材上的肝素涂层和LMWH涂层。
