Aziz D C
Cytometrics, Inc., Division of Specialty Laboratories, San Diego, California 29121-1201.
Am J Clin Pathol. 1992 Jul;98(1):105-11. doi: 10.1093/ajcp/98.1.105.
Compared to other techniques, the ability to detect estrogen and progesterone receptors by immunocytochemical analysis in formalin-fixed, paraffin-embedded sections has clear advantages, including the ability to assay small biopsy specimens, fine-needle aspirate samples, and archival material. Twenty-two cases of breast carcinoma were evaluated for estrogen and progesterone receptors by immunocytochemical analysis and enzyme immunoassay. Using a true color-based image analysis system, histograms of area versus the optical density of the positive staining nuclei were generated. A binary decision algorithm was derived from these histogram parameters by the CART computer program. Estimates generated by the algorithm for image analysis/immunocytochemical analysis had a 90% concordance with the enzyme immunoassay values. It is concluded that quantitative immunocytochemical results for estrogen and progesterone receptor content in formalin-fixed, paraffin-embedded tissue can be generated using image analysis.
与其他技术相比，在福尔马林固定、石蜡包埋切片中通过免疫细胞化学分析检测雌激素和孕激素受体的能力具有明显优势，包括能够检测小活检标本、细针穿刺抽吸样本和存档材料。通过免疫细胞化学分析和酶免疫测定对22例乳腺癌病例进行雌激素和孕激素受体评估。使用基于真彩色的图像分析系统，生成了阳性染色细胞核面积与光密度的直方图。通过CART计算机程序从这些直方图参数中导出了二元决策算法。该算法对图像分析/免疫细胞化学分析生成的估计值与酶免疫测定值有90%的一致性。结论是，使用图像分析可以得出福尔马林固定、石蜡包埋组织中雌激素和孕激素受体含量的定量免疫细胞化学结果。
