[地椒乙醇提取物对人白血病细胞系的抗肿瘤作用]
[Anti-tumor effect of ethanol extracts from Thymus quinquecostatus Celak on human leukemia cell line].
作者信息
Sun Zhen-Xiao, Zhang Ying-Hui, Cheng Shuang, Ma Qing-Wen, Guo Shan-Li, Zhang Jin-Bao
机构信息
School of Chinese Materia Medica, Beijing University of Traditional Chinese Medicine, Beijing 100102, China.
出版信息
Zhong Xi Yi Jie He Xue Bao. 2005 Sep;3(5):382-5. doi: 10.3736/jcim20050513.
OBJECTIVE
To screen the anti-tumor fraction of ethanol extracts from Thymus quinquecostatus Celak and investigate its anti-tumor effect on human leukemia cell line.
METHODS
Ethyl acetate, n-butanol and acetone fractions were separated from the ethanol extracts of wild Thymus quinquecostatus Celak. Growth inhibiting effects of these extracts on human leukemia cell lines K562 and HL-60 were determined by live cell counting and cell growth curve analysis. The possible anti-tumor mechanism was studied by morphological analysis with norcantharidin as a positive control.
RESULTS
Ethyl acetate fraction could significantly inhibit the proliferations of K562 and HL-60 cells, and the inhibiting effect depended on the concentration of ethyl acetate fraction. Ethyl acetate fraction could induce apoptosis of K562 and HL-60 cells. The n-butanol and acetone fractions had no significant inhibiting effect on K562 and HL-60 cells.
CONCLUSION
Ethyl acetate fraction is the major anti-tumor fraction in ethanol extracts from Thymus quinquecostatus Celak.
目的
筛选地椒乙醇提取物的抗肿瘤活性部位，并研究其对人白血病细胞株的抗肿瘤作用。
方法
从野生地椒乙醇提取物中分离得到乙酸乙酯部位、正丁醇部位和丙酮部位。采用活细胞计数法和细胞生长曲线分析法，测定这些提取物对人白血病细胞株K562和HL-60的生长抑制作用。以去甲斑蝥素为阳性对照，通过形态学分析研究其可能的抗肿瘤机制。
结果
乙酸乙酯部位能显著抑制K562和HL-60细胞的增殖，且抑制作用与乙酸乙酯部位的浓度有关。乙酸乙酯部位能诱导K562和HL-60细胞凋亡。正丁醇部位和丙酮部位对K562和HL-60细胞无明显抑制作用。
结论
乙酸乙酯部位是地椒乙醇提取物中的主要抗肿瘤活性部位。
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