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Hybridization with synthetic oligonucleotides.

作者信息

Szostak J W, Stiles J I, Tye B K, Chiu P, Sherman F, Wu R

出版信息

Methods Enzymol. 1979;68:419-28. doi: 10.1016/0076-6879(79)68031-x.

DOI:10.1016/0076-6879(79)68031-x
PMID:161605
Abstract
摘要

相似文献

1
Hybridization with synthetic oligonucleotides.
Methods Enzymol. 1979;68:419-28. doi: 10.1016/0076-6879(79)68031-x.
2
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Cell-free expression of large collections of cDNA clones using positive-selection lambda phage vectors.使用正选λ噬菌体载体对大量cDNA克隆进行无细胞表达。
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Gene isolation with lambda gt11 system.
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Use of synthetic oligonucleotides as hybridization probes: isolation of cloned cDNA sequences for human beta 2-microglobulin.合成寡核苷酸作为杂交探针的应用:人β2-微球蛋白克隆cDNA序列的分离
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Cloning of complementary DNA inserts from phage DNA directly into plasmid vector.将噬菌体DNA中的互补DNA插入片段直接克隆到质粒载体中。
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引用本文的文献

1
The applications of synthetic oligonucleotides to molecular biology.合成寡核苷酸在分子生物学中的应用。
Pharm Res. 1986 Aug;3(4):195-207. doi: 10.1023/A:1016382512330.
2
Single-base-pair discrimination of terminal mismatches by using oligonucleotide microarrays and neural network analyses.利用寡核苷酸微阵列和神经网络分析对末端错配进行单碱基对鉴别。
Appl Environ Microbiol. 2002 Jan;68(1):235-44. doi: 10.1128/AEM.68.1.235-244.2002.
3
Estimation of bacterial cell numbers in humic acid-rich salt marsh sediments with probes directed to 16S ribosomal DNA.
使用针对16S核糖体DNA的探针估算富腐殖酸盐沼沉积物中的细菌细胞数量。
Appl Environ Microbiol. 1999 Apr;65(4):1516-23. doi: 10.1128/AEM.65.4.1516-1523.1999.
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Use of synthetic oligonucleotide probes complementary to genes for human HLA-DR alpha and beta as extension primers for the isolation of 5'-specific genomic clones.使用与人HLA - DRα和β基因互补的合成寡核苷酸探针作为延伸引物来分离5'-特异性基因组克隆。
Proc Natl Acad Sci U S A. 1983 Mar;80(6):1531-5. doi: 10.1073/pnas.80.6.1531.
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Proc Natl Acad Sci U S A. 1982 Nov;79(22):6861-5. doi: 10.1073/pnas.79.22.6861.
7
Isolation and sequence of cDNA clones coding for a member of the family of high mobility group proteins (HMG-T) in trout and analysis of HMG-T-mRNA's in trout tissues.鳟鱼中编码高迁移率族蛋白家族成员(HMG-T)的cDNA克隆的分离与测序以及鳟鱼组织中HMG-T-mRNA的分析。
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A human and a plant intron-containing tRNATyr gene are both transcribed in a HeLa cell extract but spliced along different pathways.一个人类和一个含有内含子的植物tRNATyr基因在HeLa细胞提取物中均被转录,但沿着不同的途径进行剪接。
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