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人类补体蛋白B因子（一种III类主要组织相容性复合体基因产物）的cDNA克隆的分离

Isolation of cDNA clones for the human complement protein factor B, a class III major histocompatibility complex gene product.

作者信息

Woods D E, Markham A F, Ricker A T, Goldberger G, Colten H R

出版信息

Proc Natl Acad Sci U S A. 1982 Sep;79(18):5661-5. doi: 10.1073/pnas.79.18.5661.

DOI:10.1073/pnas.79.18.5661
PMID:6957884
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC346964/
Abstract

cDNA clones corresponding to a major histocompatibility class III antigen, the complement protein factor B, have been isolated from a human adult liver cDNA library. The clones, ranging in size from 1.0 to 2.3 kilobases, were identified by direct hybridization with two synthetic oligonucleotide mixtures. Two regions of the factor B amino acid sequence, each with minimal ambiguity in codon assignment, were chosen for synthesis of the oligonucleotides. The sequences of two clones have been partially determined. They contain coding information for the amino acid sequence of the Bb fragment of factor B and the entire 3' -untranslated region.

摘要

已从人成人肝脏cDNA文库中分离出与主要组织相容性复合体III类抗原（补体蛋白因子B）相对应的cDNA克隆。这些克隆大小在1.0至2.3千碱基之间，通过与两种合成寡核苷酸混合物直接杂交进行鉴定。选择因子B氨基酸序列的两个区域来合成寡核苷酸，每个区域在密码子分配上的歧义最小。已部分确定了两个克隆的序列。它们包含因子B的Bb片段氨基酸序列的编码信息以及整个3'非翻译区。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/844e/346964/b5a8f1563ec3/pnas00457-0241-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/844e/346964/fe024d4f4c52/pnas00457-0240-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/844e/346964/8644dc0c653c/pnas00457-0241-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/844e/346964/b5a8f1563ec3/pnas00457-0241-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/844e/346964/fe024d4f4c52/pnas00457-0240-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/844e/346964/8644dc0c653c/pnas00457-0241-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/844e/346964/b5a8f1563ec3/pnas00457-0241-b.jpg

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Isolation of cDNA clones for the human complement protein factor B, a class III major histocompatibility complex gene product.人类补体蛋白B因子（一种III类主要组织相容性复合体基因产物）的cDNA克隆的分离
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本文引用的文献

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Mouse complement components C3 and C4. Characterization of their messenger RNA and molecular cloning of complementary DNA for C3.小鼠补体成分C3和C4。它们信使RNA的特性及C3互补DNA的分子克隆。
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Identification of a precursor genomic segment that provided a sequence unique to glycophorin B and E genes.鉴定出一个前体基因组片段，该片段提供了血型糖蛋白B和E基因特有的序列。
Proc Natl Acad Sci U S A. 1993 Aug 1;90(15):7220-4. doi: 10.1073/pnas.90.15.7220.
7
Developmentally regulated effects of lipopolysaccharide on biosynthesis of the third component of complement and factor B in human fibroblasts and monocytes.脂多糖对人成纤维细胞和单核细胞中补体第三成分及B因子生物合成的发育调控作用。
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Isolation and characterisation of cDNA clones for the A alpha- and gamma-chains of human fibrinogen.人纤维蛋白原Aα链和γ链cDNA克隆的分离与鉴定
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