Inoue Hideki, Hisamoto Naoki, An Jae Hyung, Oliveira Riva P, Nishida Eisuke, Blackwell T Keith, Matsumoto Kunihiro
Department of Molecular Biology, Graduate School of Science, Institute for Advanced Research, Nagoya University, Chikusa-ku, Nagoya 464-8602, Japan.
Genes Dev. 2005 Oct 1;19(19):2278-83. doi: 10.1101/gad.1324805. Epub 2005 Sep 15.
The evolutionarily conserved p38 mitogen-activated protein kinase (MAPK) cascade is an integral part of the response to a variety of environmental stresses. Here we show that the Caenorhabditis elegans PMK-1 p38 MAPK pathway regulates the oxidative stress response via the CNC transcription factor SKN-1. In response to oxidative stress, PMK-1 phosphorylates SKN-1, leading to its accumulation in intestine nuclei, where SKN-1 activates transcription of gcs-1, a phase II detoxification enzyme gene. These results delineate the C. elegans p38 MAPK signaling pathway leading to the nucleus that responds to oxidative stress.
进化上保守的p38丝裂原活化蛋白激酶（MAPK）级联反应是对多种环境应激反应的一个组成部分。我们在此表明，秀丽隐杆线虫的PMK-1 p38 MAPK途径通过CNC转录因子SKN-1调节氧化应激反应。在氧化应激反应中，PMK-1使SKN-1磷酸化，导致其在肠细胞核中积累，在那里SKN-1激活II期解毒酶基因gcs-1的转录。这些结果描绘了秀丽隐杆线虫中导致对氧化应激作出反应的细胞核的p38 MAPK信号通路。
