Simm Roger, Fetherston Jacqueline D, Kader Abdul, Römling Ute, Perry Robert D
Department of Microbiology, Immunology, and Molecular Genetics, MS415 Medical Center, University of Kentucky, Lexington, KY 40536-0298, USA.
J Bacteriol. 2005 Oct;187(19):6816-23. doi: 10.1128/JB.187.19.6816-6823.2005.
GGDEF domain-containing proteins have been implicated in bacterial signal transduction and synthesis of the second messenger molecule cyclic-di-GMP. A number of GGDEF proteins are involved in controlling the formation of extracellular matrices. AdrA (Salmonella enterica serovar Typhimurium) and HmsT (Yersinia pestis) contain GGDEF domains and are required for extracellular cellulose production and biofilm formation, respectively. Here we show that hmsT is able to restore cellulose synthesis to a Salmonella serovar Typhimurium adrA mutant and that adrA can replace hmsT in Y. pestis Hms-dependent biofilm formation. Like Y. pestis HmsT overproducers, Y. pestis cells carrying adrA under the control of an arabinose-inducible promoter produced substantial biofilms in the presence of arabinose. Finally, we demonstrate that HmsT is involved in the synthesis of cyclic di-GMP.
含GGDEF结构域的蛋白质与细菌信号转导及第二信使分子环二鸟苷酸的合成有关。许多含GGDEF结构域的蛋白质参与细胞外基质的形成调控。AdrA（鼠伤寒沙门氏菌肠炎血清型鼠伤寒亚种）和HmsT（鼠疫耶尔森菌）含有GGDEF结构域，分别是细胞外纤维素产生和生物膜形成所必需的。在此我们表明，hmsT能够恢复鼠伤寒沙门氏菌肠炎血清型鼠伤寒亚种adrA突变体的纤维素合成，并且adrA可以替代鼠疫耶尔森菌中依赖Hms的生物膜形成过程中的hmsT。与过量表达鼠疫耶尔森菌HmsT的菌株一样，在阿拉伯糖诱导型启动子控制下携带adrA的鼠疫耶尔森菌细胞在有阿拉伯糖存在时能产生大量生物膜。最后，我们证明HmsT参与环二鸟苷酸的合成。
