Department of Food Hygiene, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Department of Parasitology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Int Microbiol. 2022 Nov;25(4):691-700. doi: 10.1007/s10123-022-00252-x. Epub 2022 Jun 8.
Acanthamoeba spp. and Salmonella share common habitats, and their interaction may influence the biofilm-forming ability of Salmonella. In this study, biofilm formation of Salmonella enterica serovar Enteritidis cocultured with Acanthamoeba castellanii was examined in nutrient-rich and nutrient-deficient media. Furthermore, transcript copy number of biofilm-related genes in the biofilm cells of S. Enteritidis in monoculture was compared to those in coculture with A. castellanii. Results demonstrated that the presence of A. castellanii in the culture media activates the genes involved in the biofilm formation of S. Enteritidis, regardless of the nutrient availability. However, biofilm formation of S. Enteritidis cocultured with A. castellanii was not consistent with the transcript copy number results. In nutrient-rich medium, the number of Salmonella biofilm cells and the contents of the three main components of the biofilms including eDNA, protein, and carbohydrates were higher in the presence of A. castellanii compared to monocultures. However, in nutrient-deficient medium, the number of biofilm cells, and the amount of biofilm components in coculture conditions were less than the monocultures. These results indicate that despite activation of relevant genes in both nutrient-rich and nutrient-deficient media, biofilm formation of S. Enteritidis cocultured with A. castellanii responds to nutrient availability.
棘阿米巴属和沙门氏菌具有共同的栖息地，它们的相互作用可能会影响沙门氏菌的生物膜形成能力。在这项研究中，研究了与棘阿米巴属共生培养的肠炎沙门氏菌在营养丰富和营养缺乏的培养基中形成生物膜的能力。此外，还比较了在单独培养和与棘阿米巴属共生培养的肠炎沙门氏菌生物膜细胞中与生物膜相关的基因的转录拷贝数。结果表明，无论营养供应情况如何，培养基中存在棘阿米巴属都会激活与肠炎沙门氏菌生物膜形成相关的基因。然而，与棘阿米巴属共生培养的肠炎沙门氏菌的生物膜形成与转录拷贝数结果不一致。在营养丰富的培养基中，与单独培养相比，存在棘阿米巴属时，肠炎沙门氏菌生物膜细胞的数量以及生物膜的三个主要成分（包括 eDNA、蛋白质和碳水化合物）的含量更高。然而，在营养缺乏的培养基中，生物膜细胞的数量以及共培养条件下生物膜成分的量均少于单独培养条件。这些结果表明，尽管在营养丰富和营养缺乏的培养基中都激活了相关基因，但与棘阿米巴属共生培养的肠炎沙门氏菌的生物膜形成会对营养供应做出响应。
