Dong Jing, Xie Chuanhui, Tian Ruijun, Wu Renan, Hu Jiwei, Zou Hanfa
National Chromatographic R&A Center, Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian, PR China.
Electrophoresis. 2005 Sep;26(18):3452-9. doi: 10.1002/elps.200500273.
Capillary electrochromatography (CEC) using a neutral hydrophobic polymer-based monolithic column has been developed for classification of analytes based on their acidity and charges of group. The monolithic columns were prepared by in situ copolymerization of lauryl methacrylate and ethylene dimethacrylate without any charged monomers in the reaction mixture. The ionic analytes will only be driven by their electrophoretic mobilities and were separated on the basis of their differences in electrophoretic mobility and in hydrophobic interaction with the stationary phase. Only negatively or positively charged analyte (acid or basic) migrated toward detection window in a single run by applying negative or positive voltage. The CEC system was also applied for the analysis of basic drugs in human serum by internal standard method using acidic running buffer. The sample of human serum spiked with basic drugs was directly injected after a simple sample pretreatment. The calibration curves with regression coefficients (0.9995-0.9997) in the range of 0.318-80 microg/mL were obtained with the limits of detection being below 0.15 microg/mL. The intra- and inter-day precisions, determined as relative standard deviations, were less than 4.21%.
已开发出一种使用基于中性疏水聚合物的整体柱的毛细管电色谱法（CEC），用于根据分析物的酸度和基团电荷对其进行分类。整体柱通过甲基丙烯酸月桂酯和二甲基丙烯酸乙二酯在反应混合物中无任何带电单体的情况下原位共聚制备。离子分析物仅由其电泳迁移率驱动，并根据其电泳迁移率以及与固定相疏水相互作用的差异进行分离。通过施加负电压或正电压，在单次运行中只有带负电荷或正电荷的分析物（酸或碱）向检测窗口迁移。CEC系统还通过使用酸性运行缓冲液的内标法用于分析人血清中的碱性药物。经过简单的样品预处理后，直接进样添加了碱性药物的人血清样品。获得了回归系数在0.9995 - 0.9997范围内、浓度范围为0.318 - 80μg/mL的校准曲线，检测限低于0.15μg/mL。以相对标准偏差确定的日内和日间精密度小于4.21%。
