Hawcroft D, Ball M, Burrows J
Department of Pharmacy, Leicester Polytechnic, United Kingdom.
Biotech Histochem. 1992 May;67(3):136-9. doi: 10.3109/10520299209110023.
Paraffin wax embedding methods suitable for demonstrating the distribution of enzyme activity in tissues sections are uncommon; most procedures rely on the use of frozen section techniques. This paper describes a system for demonstrating certain enzymes which involves incubation of the tissue with appropriate substrates before a Paramat wax embedding procedure. While it has distinct merits of its own, the procedure is eminently suitable for use where a cryostat is not available; it can also be readily applied to other enzymes and tissues.
适用于显示组织切片中酶活性分布的石蜡包埋方法并不常见；大多数程序依赖于冷冻切片技术。本文描述了一种用于显示某些酶的系统，该系统在进行Paramat蜡包埋程序之前，先将组织与合适的底物孵育。虽然该程序有其自身独特的优点，但它非常适合在没有低温恒温器的情况下使用；它也可以很容易地应用于其他酶和组织。
