Gorinstein S, Jung S-T, Moncheva S, Arancibia-Avila P, Park Y-S, Kang S-G, Goshev I, Trakhtenberg S, Namiesnik J
Department of Medicinal Chemistry and Natural Products, School of Pharmacy, The Hebrew University-Hadassah Medical School, POB 12065, Jerusalem 91120, Israel.
Arch Environ Contam Toxicol. 2005 Nov;49(4):504-10. doi: 10.1007/s00244-004-0238-2. Epub 2005 Sep 16.
Preservation of a healthy environment is a very important task, especially in the time of the total industrial revolution. Therefore, attempts to find new additional biomarkers of contamination are welcomed. For this aim, the functional and antioxidant properties of mussel Mytilus galloprovincialis proteins and the heavy metals Cd and Pb were explored. Mussels were collected in contaminated and noncontaminated sites in the Varna area of the Bulgarian Black Sea coast. Proteins were extracted from mussel entire soft tissue and analyzed using instrumental (Fourier transform infrared [FT-IR] spectroscopy, fluorescence, atomic absorption spectrophotometry), and biochemical (2,2'-azinobis 3-ethylbenzothiazoline-6-sulfonate) radical cation [ABTS(.+)]) methods. It was found that mussel proteins from the contaminated sites had specific qualitative changes: partial unfolding of the alpha-helix, slight shift in amide I bands, increased hydrophobicity, and fluorescent intensity in native and denatured samples. In the same mussel samples, an increased radical-scavenging capacity and increased contents of Cd and Pb in entire soft tissue were registered. Therefore, the above-mentioned indices could be used as additional biomarkers of sea water contamination.
保护健康的环境是一项非常重要的任务，尤其是在全面工业革命时期。因此，寻找新的额外污染生物标志物的尝试受到欢迎。为此，研究了贻贝（Mytilus galloprovincialis）蛋白质以及重金属镉（Cd）和铅（Pb）的功能和抗氧化特性。贻贝采集于保加利亚黑海沿岸瓦尔纳地区受污染和未受污染的地点。从贻贝整个软组织中提取蛋白质，并使用仪器分析方法（傅里叶变换红外光谱法、荧光法、原子吸收分光光度法）和生化方法（2,2'-联氮双（3-乙基苯并噻唑啉-6-磺酸）自由基阳离子[ABTS(·+)]）进行分析。结果发现，来自受污染地点的贻贝蛋白质有特定的定性变化：α-螺旋部分展开、酰胺I带轻微位移、疏水性增加以及天然和变性样品中的荧光强度增加。在相同的贻贝样品中，整个软组织中的自由基清除能力增强，镉和铅含量增加。因此，上述指标可作为海水污染的额外生物标志物。
