Gorinstein Shela, Moncheva Snejana, Katrich Elena, Toledo Fernando, Arancibia Patricia, Goshev Ivan, Trakhtenberg Simon
Department of Medicinal Chemistry and Natural Products, School of Pharmacy, The Hebrew University--Hadassah Medical School, P.O. Box 12065, Jerusalem 91120, Israel.
Mar Pollut Bull. 2003 Oct;46(10):1317-25. doi: 10.1016/S0025-326X(03)00239-X.
The biologically active compounds, antioxidant activities, and free radical scavenging effects of black mussel dry matter (Mytilus galloprovincialis)--(BMDM) were investigated. The extract from BMDM with absolute methanol (BMDMAMet) showed the strongest inhibition of lipid peroxidation as a function of its concentration, and was comparable to the antioxidant activity of butylated hydroxyanisole, at the same concentration of 0.2 mg/ml. The extract with 50%methanol/water (BMDM50%Met) had the weakest antioxidant activity, whereas other extracts such as 1.2 M HCl in 50%methanol/water (BMDM50%Met/HCl) exhibited an average inhibition of lipid peroxidation. BMDMAMet extract showed marked activities in free radical scavenging determined by beta-carotene bleaching (beta-carotene), nitric oxide (NO*) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) radical cation (ABTS*+ methods. The antioxidant activities of BMDMAMet extract determined beta-carotene, NO* and ABTS*+ methods showed the highest correlation coefficients (R2) such as 0.998, 0.9976 and 0.6103, respectively, with the presence of total polyphenols. BMDM50%Met had lower antioxidant ability than BMDM50%Met/HCl in different scavenging methods, indicating that the major antioxidant components in these extracts must be derived from the polyphenols. Correlation between proteins and antioxidants was very low (0.0318 and 0.0433). The mussel extracts from polluted areas have shown by all using methods higher antioxidant activity than from the clean ones. The results presented in this report indicate that the antioxidant activities of mussel extracts could possibly be an additional index of pollution characterization.
对紫贻贝干物质（Mytilus galloprovincialis）（BMDM）的生物活性化合物、抗氧化活性和自由基清除作用进行了研究。用无水甲醇提取的BMDM（BMDMAMet）提取物对脂质过氧化的抑制作用最强，且随浓度增加而增强，在0.2mg/ml相同浓度下，其抗氧化活性与丁基羟基茴香醚相当。用50%甲醇/水提取的提取物（BMDM50%Met）抗氧化活性最弱，而其他提取物如在50%甲醇/水中含1.2M盐酸的提取物（BMDM50%Met/HCl）对脂质过氧化有中等程度的抑制作用。通过β-胡萝卜素漂白法（β-胡萝卜素）、一氧化氮（NO*）和2,2'-联氮-双-（3-乙基苯并噻唑啉-6-磺酸）自由基阳离子（ABTS*+法）测定，BMDMAMet提取物在自由基清除方面表现出显著活性。用β-胡萝卜素、NO和ABTS+法测定BMDMAMet提取物的抗氧化活性，与总多酚含量的相关系数（R2）最高，分别为0.998、0.9976和0.6103。在不同的清除方法中，BMDM50%Met的抗氧化能力低于BMDM50%Met/HCl，表明这些提取物中的主要抗氧化成分一定来自多酚。蛋白质与抗氧化剂之间的相关性非常低（0.0318和0.0433）。所有使用的方法都表明，来自污染区域的贻贝提取物比来自清洁区域的具有更高的抗氧化活性。本报告中的结果表明，贻贝提取物的抗氧化活性可能是污染特征的一个附加指标。
