Tandon V R, Gupta R K
Post Graduate Department of Pharmacology & Therapeutics, Govt. Medical College, Jammu 180 001.
Indian J Physiol Pharmacol. 2005 Apr;49(2):199-205.
Maximal electroshock seizures (MES) in albino rats and pentylenetetarazole (PTZ) induced seizures in albino mice were used to study anticonvulsant activity of Vitex-negundo leaf extract. The ethanolic leaf extract of Vitex-negundo was administered orally in graded doses (250, 500 and 1000 mg/kg p.o) in both the experimental models and the effects were compared with diphenylhydantoin in MES method and valporic acid in PTZ induced seizures method as standard control respectively. The Vitex-negundo in the doses (250, 500 and 1000 mg/kg, p.o) did not show protection against MES to any significant extent but significant post-ictal depression was observed in the dose of 1000 mg/kg body weight in comparison to control. However, sub-protective dose of test drug (100 mg/ kg, p.o) potentiated the anticonvulsant action of diphenylhydantoin. The test drug in the dose (1000 mg/kg, po) showed 50% protection in clonic seizures and 24-hour mortality against PTZ induced seizures. It also decreased number and duration of convulsions significantly. Vitex-negundo potentiated anticonvulsant activity of valporic acid. The anticonvulsant activity of Vitex-negundo has not been found equi-effective with standard drugs. These findings suggest that Vitex-negundo possesses anticonvulsant activity particularly against PTZ induced convulsions. Moreover, the potentiation of diphenylhydantoin and valporic acid by Vitex-negundo indicates that it may be useful as an adjuvant therapy along with standard anticonvulsants and can possibly lower the requirement of diphenylhydantoin and valporic acid.
采用白化大鼠最大电休克发作（MES）和白化小鼠戊四氮（PTZ）诱导的惊厥来研究蔓荆子叶提取物的抗惊厥活性。在两种实验模型中，均以分级剂量（250、500和1000 mg/kg口服）给予蔓荆子乙醇叶提取物，并分别将其效果与MES法中的苯妥英钠和PTZ诱导惊厥法中的丙戊酸作为标准对照进行比较。蔓荆子剂量为250、500和1000 mg/kg口服时，在很大程度上未显示出对MES的保护作用，但与对照组相比，在体重1000 mg/kg剂量下观察到明显的发作后抑郁。然而，受试药物的亚保护剂量（100 mg/kg口服）增强了苯妥英钠的抗惊厥作用。受试药物剂量为1000 mg/kg口服时，对PTZ诱导的惊厥在阵挛性发作中显示出50%的保护作用和24小时死亡率。它还显著减少了惊厥的次数和持续时间。蔓荆子增强了丙戊酸的抗惊厥活性。尚未发现蔓荆子的抗惊厥活性与标准药物等效。这些发现表明，蔓荆子具有抗惊厥活性，尤其是对PTZ诱导的惊厥。此外，蔓荆子对苯妥英钠和丙戊酸的增强作用表明，它可能作为标准抗惊厥药物的辅助治疗有用，并且可能降低苯妥英钠和丙戊酸的需求量。
