Gupta R K, Tandon V R
Department of Pharmacology, M.G.I.M.S. (Sevagram), Wardha 442 102.
Indian J Physiol Pharmacol. 2005 Apr;49(2):163-70.
Tail flick test in rats and acetic acid induced writhing in mice were employed to study the antinociceptive activity of ethanolic leaf extract of Vitex-negundo (VN) (100, 250 and 500 mg/kg, p.o). The effect was compared with meperidine (40 mg/kg, sc) in tail flick method and aspirin (50 mg/kg, p.o) in writhing test as a standard control respectively. An interaction with naloxone hydrochloride was also studied in tail flick method for its mechanism of central analgesic action. The test drug showed significant analgesic activity in dose dependant manner in both the experimental models. In comparison to standard drug (meperidine), more than ten times dose of VN extract was required to produce comparable significant antinociceptive activity. The sub-effective dose (5 mg/kg, po) of VN potentiated the analgesic activity of meperidine (4 mg/kg, sc) and aspirin (25 mg/kg, po). Naloxone (1 mg/kg, sc) did not reverse the analgesic effect of VN extract. Our observations suggest that VN possesses both central and peripheral analgesic activity. The central analgesic action does not seem to be mediated through opioid receptors. It, may prove to be a useful adjuvant therapy along with standard analgesic drug.
采用大鼠甩尾试验和小鼠醋酸诱导扭体试验，研究了黄荆（VN）乙醇叶提取物（100、250和500毫克/千克，口服）的抗伤害感受活性。分别将甩尾试验中其效果与哌替啶（40毫克/千克，皮下注射）以及扭体试验中阿司匹林（50毫克/千克，口服）作为标准对照进行比较。还在甩尾试验中研究了与盐酸纳洛酮的相互作用，以探讨其中枢镇痛作用机制。受试药物在两种实验模型中均呈剂量依赖性地显示出显著的镇痛活性。与标准药物（哌替啶）相比，需要超过十倍剂量的VN提取物才能产生相当的显著抗伤害感受活性。VN的亚有效剂量（5毫克/千克，口服）增强了哌替啶（4毫克/千克，皮下注射）和阿司匹林（25毫克/千克，口服）的镇痛活性。纳洛酮（1毫克/千克，皮下注射）并未逆转VN提取物的镇痛作用。我们的观察结果表明，VN具有中枢和外周镇痛活性。中枢镇痛作用似乎不是通过阿片受体介导的。它可能被证明是一种与标准镇痛药联合使用的有用辅助疗法。
