Vieira A, Overweg E, Ruben J L, Huysmans M C D N J M
Department of Dentistry and Dental Hygiene, University Medical Centre Groningen, A. Deusinglaan 1, 9713 AV Groningen, The Netherlands.
J Dent. 2006 May;34(5):336-42. doi: 10.1016/j.jdent.2005.07.010. Epub 2005 Sep 19.
Enamel erosion results in the formation of a softened layer that is susceptible to disruption by mechanical factors such as brushing abrasion, tongue friction and attrition. The aim of this study was to investigate the individual contribution of those mechanical insults to the enamel loss caused by dental erosion.
Forty two bovine enamel samples were randomly divided into seven groups (n=6 per group) that were submitted to 3cycles of one of the following regimes: erosion and remineralization (er/remin); toothbrush abrasion and remineralization (abr/remin); erosion, toothbrush abrasion and remineralization (er/abr/remin); attrition and remineralization (at/remin); erosion, attrition and remineralization (er/at/remin); simulated tongue friction and remineralization (tg/remin); erosion, simulated tongue friction and remineralization (er/tg/remin). Erosion took place in a demineralization solution (50mM citric acid, pH 3) for 10min under agitation. Brushing abrasion, tongue friction and attrition were simulated for 1min using a home-made wear device. Remineralization was carried out in artificial saliva for 2h. Enamel loss was quantified using optical profilometry.
One-way ANOVA indicated a significant difference between the amounts of enamel lost due to the different wear regimes (p<or=0.001). Multiple comparisons with Bonferroni procedure showed that the wear depths found for the er/at/remin (p<or=0.001) and er/tg/remin (p<or=0.001) were significantly higher than the amount found for the er/remin group (4.4+/-0.7microm). This was not the case for er/abr/remin group (p=0.075).
The results suggest that the three forms of mechanical insults remove to varying extent the softened layer formed by the erosion challenges.
牙釉质侵蚀会导致形成一层软化层，该软化层易受刷牙磨损、舌摩擦和磨耗等机械因素的破坏。本研究的目的是调查这些机械损伤对牙釉质侵蚀所致牙釉质丧失的个体影响。
42个牛牙釉质样本被随机分为7组（每组n = 6），分别接受以下一种方案的3个循环：侵蚀和再矿化（er/remin）；牙刷磨损和再矿化（abr/remin）；侵蚀、牙刷磨损和再矿化（er/abr/remin）；磨耗和再矿化（at/remin）；侵蚀、磨耗和再矿化（er/at/remin）；模拟舌摩擦和再矿化（tg/remin）；侵蚀、模拟舌摩擦和再矿化（er/tg/remin）。侵蚀在脱矿溶液（50mM柠檬酸，pH 3）中搅拌10分钟进行。使用自制磨损装置模拟刷牙磨损、舌摩擦和磨耗1分钟。再矿化在人工唾液中进行2小时。使用光学轮廓仪对牙釉质丧失进行定量。
单因素方差分析表明，不同磨损方案导致的牙釉质丧失量之间存在显著差异（p≤0.001）。采用Bonferroni法进行的多重比较显示，er/at/remin组（p≤0.001）和er/tg/remin组（p≤0.001）的磨损深度显著高于er/remin组（4.4±0.7微米）。er/abr/remin组情况并非如此（p = 0.075）。
结果表明，这三种形式的机械损伤在不同程度上去除了由侵蚀挑战形成的软化层。
