Yamahata Christophe, Vandevyver Caroline, Lacharme Frédéric, Izewska Paulina, Vogel Horst, Freitag Ruth, Gijs Martin A M
Institute of Microelectronics and Microsystems, Ecole Polytechnique Fédérale de Lausanne (EPFL), Switzerland.
Lab Chip. 2005 Oct;5(10):1083-8. doi: 10.1039/b504468e. Epub 2005 Aug 23.
We discuss the successful transport of jurkat cells and 5D10 hybridoma cells using a reciprocating micropump with nozzle-diffuser elements. The effect of the pumping action on cell viability and proliferation, as well as on the damaging of cellular membranes is quantified using four types of well-established biological tests: a trypan blue solution, the tetrazolium salt WST-1 reagent, the LDH cytotoxicity assay and the calcium imaging ATP test. The high viability levels obtained after pumping, even for the most sensitive cells (5D10), indicate that a micropump with nozzle-diffuser elements can be very appropriate for handling living cells in cell-on-a-chip applications.
我们讨论了使用带有喷嘴-扩散器元件的往复式微泵成功运输Jurkat细胞和5D10杂交瘤细胞的情况。使用四种成熟的生物学测试对泵送作用对细胞活力、增殖以及细胞膜损伤的影响进行了量化：台盼蓝溶液、四唑盐WST-1试剂、乳酸脱氢酶（LDH）细胞毒性测定和钙成像ATP测试。泵送后获得的高活力水平，即使对于最敏感的细胞（5D10）也是如此，这表明带有喷嘴-扩散器元件的微泵非常适合在芯片上细胞应用中处理活细胞。
