Kurose K, Iida A, Araki T, Sakamoto G, Kasumi F, Nakamura Y, Emi M
Department of Molecular Biology, Nippon Medical School, Kawasaki.
Jpn J Cancer Res. 1998 May;89(5):533-8. doi: 10.1111/j.1349-7006.1998.tb03294.x.
We examined 142 primary human breast cancers to determine their patterns of loss of heterozygosity (LOH) at 19 microsatellite markers over the entire length of chromosome 7. Allelic loss at one or more loci on the short arm of chromosome 7 was observed in 37 of the tumors (26%). We found a new target region of allelic loss on 7p between D7S1802 and D7S817 at 7p14-15. LOH on 7p was found more frequently in tumors of the invasive solid tubular and scirrhous type (31 of 87); 36%) than in other less aggressive types (2 of 27; 7%) (P = 0.0047). The results suggest that inactivation of putative tumor suppressor gene(s) located at 7p14-15 may play a role in the development and/or progression of primary breast cancers, particularly those of the invasive solid tubular and scirrhous type. Allelic loss was also found in 56 of 142 tumors on the long arm, and a commonly deleted region was defined between D7S522 and D7S1801 at 7q31.
我们检测了142例原发性人类乳腺癌，以确定其在7号染色体全长19个微卫星标记处的杂合性缺失（LOH）模式。在37例肿瘤（26%）中观察到7号染色体短臂上一个或多个位点的等位基因缺失。我们在7p14 - 15的D7S1802和D7S817之间发现了7p上等位基因缺失的一个新的靶区域。7p上的LOH在浸润性实性管状和硬癌类型的肿瘤中更常见（87例中有31例；36%），而在其他侵袭性较小的类型中较少见（27例中有2例；7%）（P = 0.0047）。结果表明，位于7p14 - 15的假定肿瘤抑制基因的失活可能在原发性乳腺癌尤其是浸润性实性管状和硬癌类型的发生和/或进展中起作用。在142例肿瘤的56例中还发现了7号染色体长臂上的等位基因缺失，并且在7q31的D7S522和D7S1801之间确定了一个常见的缺失区域。
