Wilson D F, Cerniglia G J
Department of Biochemistry, Medical School, University of Pennsylvania, Philadelphia 19104.
Cancer Res. 1992 Jul 15;52(14):3988-93.
Oxygen-dependent quenching of phosphorescence has been used to image the distribution of oxygen pressure in small tumors and surrounding tissue. Suspensions of cultured 9L cells or small pieces of solid tumors from 9L cells were injected into the surface of the muscle of the hindquarter of rats, and the tumors were grown until they were 0.2-1.0 cm in diameter. The phosphorescent probe for oxygen was injected into the systemic blood, and phosphorescence was imaged with a video camera. Images of the phosphorescence were collected using a series of different delay times after illumination with a light flash (less than 5-microseconds width at half-height), and the phosphorescence decay constants (lifetimes) and oxygen pressure were calculated for each pixel of the image arrays. The areas of tissue within the tumors were observed to have increased phosphorescence lifetimes and lower oxygen pressures than the surrounding tissue. Phosphorescence imaging is, therefore, a noninvasive optical method which permits quantitation of the distribution of oxygen in small tumors and also, at least in the 9L tumors, differentiation of tumor from normal tissue.
磷光的氧依赖猝灭已被用于对小肿瘤及周围组织中的氧压分布进行成像。将培养的9L细胞悬液或来自9L细胞的小块实体瘤注射到大鼠后肢肌肉表面，使肿瘤生长至直径为0.2 - 1.0厘米。将氧磷光探针注入体循环血液中，并用摄像机对磷光进行成像。在用闪光（半高宽小于5微秒）照射后，使用一系列不同的延迟时间收集磷光图像，并为图像阵列的每个像素计算磷光衰减常数（寿命）和氧压。观察到肿瘤内组织区域的磷光寿命增加，氧压低于周围组织。因此，磷光成像是非侵入性光学方法，可对小肿瘤中的氧分布进行定量，并且至少在9L肿瘤中能区分肿瘤组织与正常组织。
