Watanabe S, Sasaki J
Department of Anatomy, Okayama University Medical School, Japan.
Cell Tissue Res. 1992 May;268(2):239-45. doi: 10.1007/BF00318792.
12-O-tetradecanoylphorbol-13-acetate (TPA) is known to affect the proliferation and/or differentiation of several types of cells. We injected TPA directly into the lumen of rat bladder to determine, using scanning and transmission electron microscopy, its effects on the bladder epithelium in vivo. At 1 h after TPA injection (1 microgram/ml), the superficial cells of the epithelium had changed their morphology, and large spherical vacuoles occupied their cytoplasm. In some areas, the underlying intermediate cells were exposed by the desquamation of the superficial cells. During the next few hours, TPA was excreted from the bladder lumen by voluntary micturition, but the desquamation of the superficial cells proceeded further. All the superficial cells were lost from the luminal surface by 24 h after TPA injection. The changes noted were specific for the superficial cells and were not observed in the intermediate or basal cells. After 24 h, part of the epithelium had a three-layer structure, indicating that regeneration was taking place. These results demonstrate that TPA selectively affects and desquamates superficial cells in a short period of time. This experimental system may be useful for studying in vivo cell proliferation and/or differentiation.
已知12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）会影响多种类型细胞的增殖和/或分化。我们将TPA直接注入大鼠膀胱腔内，通过扫描电子显微镜和透射电子显微镜来确定其对体内膀胱上皮的影响。在注射TPA（1微克/毫升）后1小时，上皮的表层细胞形态发生改变，其细胞质中充满了大的球形空泡。在一些区域，表层细胞的脱屑使下方的中间细胞暴露出来。在接下来的几个小时里，TPA通过自主排尿从膀胱腔中排出，但表层细胞的脱屑仍进一步发展。在注射TPA后24小时，腔表面的所有表层细胞都消失了。所观察到的变化是表层细胞特有的，在中间细胞或基底细胞中未观察到。24小时后，部分上皮具有三层结构，表明正在发生再生。这些结果表明，TPA在短时间内选择性地影响并使表层细胞脱屑。该实验系统可能有助于研究体内细胞增殖和/或分化。
