Skouv J, Christensen B, Skibshøj I, Autrup H
Carcinogenesis. 1986 Feb;7(2):331-3. doi: 10.1093/carcin/7.2.331.
The effect of a single treatment with the skin tumor-promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) on the expression of the cellular proto-oncogenes, c-myc, c-rasHa, c-rasKi and c-fos was examined in the non-tumorigenic human bladder epithelial cell line HCV 29. TPA (1 microgram/ml) increased the transcription of the c-fos gene of HCV 29 at least 50-fold, and this stimulation was observed within minutes. The response was transient, and was accompanied by a rapid and transient change in cell morphology. The expression of c-myc, c-rasHa and c-rasKi were not enhanced by the TPA treatment. These results show that human bladder epithelial cells respond to a known skin tumor-promoter, TPA, by altering the transcription of a specific proto-oncogene in these cells.
在非致瘤性人膀胱上皮细胞系HCV 29中，研究了用皮肤肿瘤促进剂12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）单次处理对细胞原癌基因c - myc、c - rasHa、c - rasKi和c - fos表达的影响。TPA（1微克/毫升）使HCV 29的c - fos基因转录增加至少50倍，且在数分钟内即可观察到这种刺激。该反应是短暂的，并伴有细胞形态的快速且短暂变化。TPA处理未增强c - myc、c - rasHa和c - rasKi的表达。这些结果表明，人膀胱上皮细胞通过改变这些细胞中特定原癌基因的转录来响应已知的皮肤肿瘤促进剂TPA。
