Jensen A T, Gasim S, Moller T, Ismail A, Gaafar A, Kemp M, el Hassan A M, Kharazmi A, Alce T M, Smith D F, Theander T G
Centre for Medical Parasitology at Institute for Medical Microbiology and Immunology, University of Copenhagen, Denmark.
Trans R Soc Trop Med Hyg. 1999 Mar-Apr;93(2):157-60. doi: 10.1016/s0035-9203(99)90291-2.
The repetitive sequence of Leishmania major gene B protein (GBP) has previously been shown to be a useful tool in the diagnosis of cutaneous leishmaniasis (CL). Here, we have assessed enzyme-linked immunosorbent assays (ELISAs) using recombinant L. donovani GBP (rGBP) and a peptide sequence of L. donovani GBP (GBPP) in the diagnosis of L. donovani infections in Sudan. The sensitivity of the rGBP ELISA in diagnosing visceral leishmaniasis (VL) and post kala-azar dermal leishmaniasis (PKDL) was 92% and 93%, respectively. In contrast, the sensitivity of the GBPP ELISA was 55% for VL and 63% for PKDL. Plasma antibody reactivity of donors with VL and PKDL remained high for an extended period after the end of treatment. Antibody-reactivity to rGBP and GBPP was detected in 71% and 14% of plasma samples from CL patients, respectively. Plasma from healthy Sudanese donors living in an area endemic for malaria but free of leishmaniasis was negative in both assays.
硕大利什曼原虫基因B蛋白（GBP）的重复序列先前已被证明是诊断皮肤利什曼病（CL）的一种有用工具。在此，我们评估了使用重组杜氏利什曼原虫GBP（rGBP）和杜氏利什曼原虫GBP的肽序列（GBPP）的酶联免疫吸附测定（ELISA）在苏丹诊断杜氏利什曼原虫感染中的应用。rGBP ELISA诊断内脏利什曼病（VL）和黑热病后皮肤利什曼病（PKDL）的敏感性分别为92%和93%。相比之下，GBPP ELISA诊断VL的敏感性为55%，诊断PKDL的敏感性为63%。VL和PKDL患者治疗结束后，其血浆抗体反应性在较长时间内仍保持较高水平。CL患者的血浆样本中，分别有71%和14%检测到对rGBP和GBPP的抗体反应性。生活在疟疾流行但无利什曼病地区的健康苏丹献血者的血浆在两种检测中均为阴性。
