Miyata K, Kato M, Shikama H, Nishimura K, Sakae N, Kawagoe K, Nishikawa T, Kuroda K, Yamaguchi K, Aoyama Y
Biochemistry Research Laboratory, Central Research Institute, Shiga, Japan.
Clin Exp Metastasis. 1992 Jul;10(4):267-72. doi: 10.1007/BF00133562.
The injection of B16F10 melanoma cells with recombinant human tumor necrosis factor alpha (TNF-alpha) into the tail vein of C57BL/6 mice resulted in 2- to 25-fold more metastatic foci in the lungs than the injection of tumor cells alone. Clearly, TNF-alpha significantly enhanced experimental tumor metastasis. Furthermore, it enhanced the metastasis of Lewis lung carcinoma cells. In contrast, a mutein of TNF-alpha, designated as F4236, having the cell-adhesive sequence (Tyr-Ile-Gly-Ser-Arg) at the N-terminus of the TNF molecule did not enhance metastasis, but rather exhibited similar antitumor activity to wild-type TNF-alpha in fibrosarcoma-bearing mice.
将携带重组人肿瘤坏死因子α（TNF-α）的B16F10黑色素瘤细胞注射到C57BL/6小鼠的尾静脉中，与单独注射肿瘤细胞相比，肺部的转移灶多出2至25倍。显然，TNF-α显著增强了实验性肿瘤转移。此外，它还增强了Lewis肺癌细胞的转移。相比之下，一种TNF-α的突变体，命名为F4236，在TNF分子的N端具有细胞黏附序列（酪氨酸-异亮氨酸-甘氨酸-丝氨酸-精氨酸），它并没有增强转移，而是在携带纤维肉瘤的小鼠中表现出与野生型TNF-α相似的抗肿瘤活性。
