Chen Jian, Li Ming, Shu Yan-Wen, Liao Yu-Hua
Department of Cardiovasology, Union Hospital, Tongji Medical College, Huazhong Science and Technology University, Wuhan 430022, China.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2005 Aug;27(4):470-4.
To investigate effects of supplementation of folate on the expression of monocyte chemoattractant protein-1 (MCP-1) in aortic endothelium and release from peripheral blood mononuclear cells (PBMC) in rats with hyperhomocysteinemia induced by ingestion of excess methionine.
Thirty male SD rats were randomly divided into 3 groups (n = 10 for each group): control group (Control), high homocysteinemia group (Hhcy), and folate supplementation group (FA). They were fed with nomal diet, normal diet enriched by 1.7% methionine, and normal diet plus 1.7% methionine and 0.006% folate, respectively, for 45 days. The levels of total plasma homocysteine (Thcy) were measured by high performance liquid chromatography and the concentrations of chemokine MCP-1 released from PBMC stimulated by oxidized low density lipoprotein were detected by enzyme immunoassays. The expression of MCP-1 on aortas of rats was detected by immunohistochemistry and Western blot.
A high methionine diet for 45 days induced hyperhomocysteinemia. Folate supplementation to high-methionine diet significantly decreased plasma Thcy levels (P < 0.01). The expression of MCP-1 in aortic endothelium and the levels of MCP-1 released from PBMC stimulated by oxidized low density lipoprotein were significantly higher in rats of Hhcy group than in rats of control group (P < 0.05, P < 0.01). During supplementation of folate, normalization of Thcy levels was accompanied by a marked reduction of MCP-1 expression in aortic endothelium and by a significant decrease of MCP-1 released from PBMC stimulated by oxidized low density lipoprotein (P < 0.05, P < 0.01).
Folate supplementation can prevent an elevation of homocysteine levels in the blood and decrease the expression MCP-1 in aortic endothelium and release of MCP-1 from PBMC in rats with hyperhomocysteinemia.
探讨补充叶酸对过量摄入蛋氨酸诱导的高同型半胱氨酸血症大鼠主动脉内皮中单核细胞趋化蛋白-1（MCP-1）表达及外周血单个核细胞（PBMC）释放MCP-1的影响。
将30只雄性SD大鼠随机分为3组（每组n = 10）：对照组（Control）、高同型半胱氨酸血症组（Hhcy）和叶酸补充组（FA）。分别给予正常饮食、添加1.7%蛋氨酸的正常饮食以及添加1.7%蛋氨酸和0.006%叶酸的正常饮食，持续45天。采用高效液相色谱法测定血浆总同型半胱氨酸（Thcy）水平，用酶免疫分析法检测氧化型低密度脂蛋白刺激PBMC释放的趋化因子MCP-1浓度。通过免疫组织化学和蛋白质印迹法检测大鼠主动脉中MCP-1的表达。
45天的高蛋氨酸饮食诱导了高同型半胱氨酸血症。高蛋氨酸饮食中补充叶酸可显著降低血浆Thcy水平（P < 0.01）。Hhcy组大鼠主动脉内皮中MCP-1的表达以及氧化型低密度脂蛋白刺激PBMC释放的MCP-1水平显著高于对照组大鼠（P < 0.05，P < 0.01）。在补充叶酸期间，Thcy水平恢复正常的同时，主动脉内皮中MCP-1的表达显著降低，氧化型低密度脂蛋白刺激PBMC释放的MCP-1也显著减少（P < 0.05，P < 0.01）。
补充叶酸可预防高同型半胱氨酸血症大鼠血液中同型半胱氨酸水平升高，并降低主动脉内皮中MCP-1的表达以及PBMC中MCP-1的释放。
