Ghannoum M A, Moussa N M, Whittaker P, Swairjo I, Abu-Elteen K H
Division of Infectious Diseases, LAC Harbor-UCLA Medical Center, UCLA School of Medicine, Torrance.
Chemotherapy. 1992;38(1):46-56. doi: 10.1159/000238941.
The effect of subinhibitory concentrations of octenidine and pirtenidine on the lipid and sterol composition of Candida albicans was investigated. The total lipid and sterol contents of C. albicans grown in the presence of either octenidine or pirtenidine were reduced compared with control-grown cells. The major differences in the lipid composition of drug-grown and control cells were phosphatidylglycerol, phosphatidylcholine and monogalactosyldiacylglycerol, which increased in the presence of octenidine and pirtenidine. Lower proportions of phosphatidic acid were found in yeasts grown in the presence of the drugs when compared with control C. albicans. Fatty acid analysis of control-grown cells showed that the major fatty acids were C16 and C18. Drug-grown cells had higher proportions of palmitic and linolenic acids but lower proportion of oleic acid. The C16/C18 ratios were higher for octenidine- and pirtenidine-grown cells than control cells. Differences in the fatty acid composition of major phospholipids and neutral lipids between drug-grown and control yeasts were also observed. Sterol analysis of control-grown cells showed that the major sterol present was ergosterol (65.9%). A significant increase in squalene and 4,14-dimethylzymosterol was observed in pirtenidine-treated cells, while octenidine-treated cells showed an increase in zymosterol and obtusifoliol contents. Our results suggest that octenidine and pirtenidine affect the lipids and sterol of C. albicans in different ways. The implications of these findings on the mode of action of these two drugs is discussed.
研究了亚抑菌浓度的奥替尼啶和匹替尼啶对白色念珠菌脂质和甾醇组成的影响。与对照生长的细胞相比，在奥替尼啶或匹替尼啶存在下生长的白色念珠菌的总脂质和甾醇含量降低。药物处理组和对照组细胞脂质组成的主要差异在于磷脂酰甘油、磷脂酰胆碱和单半乳糖基二酰基甘油，它们在奥替尼啶和匹替尼啶存在下增加。与对照白色念珠菌相比，在药物存在下生长的酵母中磷脂酸的比例较低。对照生长细胞的脂肪酸分析表明，主要脂肪酸为C16和C18。药物处理组细胞中棕榈酸和亚麻酸的比例较高，但油酸的比例较低。奥替尼啶和匹替尼啶处理组细胞的C16/C18比值高于对照细胞。还观察到药物处理组酵母和对照酵母之间主要磷脂和中性脂质的脂肪酸组成存在差异。对照生长细胞的甾醇分析表明，主要存在的甾醇是麦角甾醇（65.9%）。在匹替尼啶处理的细胞中观察到角鲨烯和4,14-二甲基酵母甾醇显著增加，而奥替尼啶处理的细胞中酵母甾醇和钝叶醇含量增加。我们的结果表明，奥替尼啶和匹替尼啶以不同方式影响白色念珠菌的脂质和甾醇。讨论了这些发现对这两种药物作用模式的影响。
