Cox M J, Elwin K, Massad E, Azevedo R S
Biological and Molecular Sciences, School of Science and the Environment, Coventry University, Coventry, UK.
Epidemiol Infect. 2005 Oct;133(5):951-6. doi: 10.1017/S0950268805004036.
The seroepidemiology of Cryptosporidium infection was investigated in a representative sample of a normal population in the State of Sao Paulo, Brazil using a recombinant form of the immunodominant 27-kDa sporozoite antigen. IgG seropositivity was low in infants following loss of maternal antibody but quickly increased to approximately 60% by 5 years, then 80% by the age of 10 years, after which prevalence remained constant. The broad range of antibody concentrations is consistent with previous reports that the IgG response to C. parvum is short-lived. There is also evidence that average antibody concentrations increase with age. Results suggest that the recombinant antigen may be a more sensitive method of measuring seroprevalence than the native antigen in Western blot. Although cross-sectional studies can provide an insight into the epidemiology of C. parvum in normal populations, further studies investigating the dynamics of the humoral immune responses to Cryptosporidium and the use of serology in epidemiological studies are required.
利用重组形式的免疫显性27 kDa子孢子抗原，对巴西圣保罗州正常人群的代表性样本进行了隐孢子虫感染的血清流行病学调查。婴儿在母体抗体消失后IgG血清阳性率较低，但到5岁时迅速升至约60%，到10岁时升至80%，此后患病率保持稳定。抗体浓度范围广泛与先前关于对微小隐孢子虫的IgG反应短暂的报道一致。也有证据表明平均抗体浓度随年龄增长而增加。结果表明，在蛋白质印迹法中，重组抗原可能是一种比天然抗原更敏感的血清流行率检测方法。虽然横断面研究可以深入了解正常人群中微小隐孢子虫的流行病学，但还需要进一步研究隐孢子虫体液免疫反应的动态变化以及血清学在流行病学研究中的应用。
