Gueguen E, Rousseau P, Duval-Valentin G, Chandler M
Laboratoire de Microbiologie et Génétique Moléculaires, CNRS UMR5100, 118 Route de Narbonne, 31062 Toulouse Cedex, France.
Trends Microbiol. 2005 Nov;13(11):543-9. doi: 10.1016/j.tim.2005.09.002. Epub 2005 Sep 21.
Studies of several transposable genetic elements have pinpointed the importance of the transpososome, a nucleoprotein complex involving the transposon ends and a transposon-encoded enzyme--the transposase--as a key in regulating transposition. Transpososomes provide a precise architecture within which the chemical reactions involved in transposon displacement occur. Data are accumulating that suggest they are dynamic and undergo staged conformational changes to accommodate different steps in the transposition pathway. This has been underpinned by recent results obtained particularly with Tn5, Tn10 and bacteriophage Mu.
对几种转座遗传元件的研究已经明确了转座体的重要性，转座体是一种核蛋白复合体，涉及转座子末端和一种转座子编码的酶——转座酶，它是调节转座的关键。转座体提供了一个精确的结构，转座子移位所涉及的化学反应在其中发生。越来越多的数据表明，它们是动态的，会经历阶段性的构象变化以适应转座途径中的不同步骤。这一点已得到最近特别是对Tn5、Tn10和噬菌体Mu的研究结果的支持。
