Romão Célia Maria Carvalho Pereira Araujo, Faria Yaisa Naziozeno de, Pereira Luciana Roberto, Asensi Marise Dutra
Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ, 21040-900, Brazil.
Mem Inst Oswaldo Cruz. 2005 Aug;100(5):541-8. doi: 10.1590/s0074-02762005000500015. Epub 2005 Sep 15.
The aim of this study was to evaluate the susceptibility of 35 resistant Pseudomonas aeruginosa clinical isolates to a quaternary ammonium hospital disinfectant. The methodology was the AOAC Use-Dilution Test, with disinfectant at its use-concentration. In addition, the chromosomal DNA profile of the isolates were determined by macro-restriction pulsed field gel electrophoresis (PFGE) method aiming to verify the relatedness among them and the behavior of isolates from the same group regarding the susceptibility to the disinfectant. Seventy one percent of the isolates were multiresistant to antibiotics and 43% showed a reduced susceptibility to the disinfectant. The PFGE methodology detected 18 major clonal groups. We found isolates with reduced susceptibility to the disinfectant and we think that these are worrying data that should be further investigated including different organisms and chemical agents in order to demonstrate that microorganisms can be destroyed by biocide as necessary. We also found strains of the same clonal groups showing different susceptibility to the disinfectant. This is an interesting observation considering that only few works are available about this subject. PFGE profile seems not to be a reliable marker for resistance to disinfectants.
本研究的目的是评估35株耐多药铜绿假单胞菌临床分离株对一种季铵类医院消毒剂的敏感性。采用AOAC使用稀释试验方法，使用消毒剂的使用浓度。此外，通过宏观限制性脉冲场凝胶电泳（PFGE）方法确定分离株的染色体DNA图谱，旨在验证它们之间的相关性以及同一组分离株对消毒剂的敏感性表现。71%的分离株对多种抗生素耐药，43%的分离株对消毒剂的敏感性降低。PFGE方法检测到18个主要克隆群。我们发现了对消毒剂敏感性降低的分离株，我们认为这些令人担忧的数据应进一步研究，包括不同的微生物和化学试剂，以证明微生物可根据需要被杀菌剂杀灭。我们还发现同一克隆群的菌株对消毒剂的敏感性不同。考虑到关于这个主题的研究很少，这是一个有趣的观察结果。PFGE图谱似乎不是消毒剂耐药性的可靠标志物。
