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胰岛淀粉样多肽（IAPP）在胰岛中合成。通过原位杂交和免疫组化相结合的方法在大鼠胰腺中检测IAPP多肽和IAPP mRNA。

Islet amyloid polypeptide (IAPP) is synthesized in the islets of Langerhans. Detection of IAPP polypeptide and IAPP mRNA by combined in situ hybridization and immunohistochemistry in rat pancreas.

作者信息

Denijn M, De Weger R A, Van Mansfeld A D, van Unnik J A, Lips C J

机构信息

Department of Pathology, University Hospital Utrecht, The Netherlands.

出版信息

Histochemistry. 1992;97(1):33-7. doi: 10.1007/BF00271279.

DOI:10.1007/BF00271279
PMID:1618637
Abstract

We investigated the localization of IAPP mRNA by means of in situ hybridization in tissue sections of rat pancreas. A 35S-labeled, IAPP-specific DNA probe--hybridized specifically in the islets of Langerhans. This localization was confirmed by immunohistochemical localization of insulin and IAPP polypeptides on adjacent tissue sections. Moreover, combined in situ hybridization of IAPP mRNA and immunohistochemistry of insulin and IAPP polypeptide on the same section, using insulin as specific marker shows the presence of IAPP mRNA in the islets of Langerhans.

摘要

我们通过原位杂交技术在大鼠胰腺组织切片中研究了胰岛淀粉样多肽（IAPP）mRNA的定位。一个用35S标记的、IAPP特异性DNA探针在胰岛中特异性杂交。胰岛素和IAPP多肽在相邻组织切片上的免疫组织化学定位证实了这种定位。此外，在同一切片上使用胰岛素作为特异性标记物，对IAPP mRNA进行原位杂交与胰岛素和IAPP多肽进行免疫组织化学相结合的方法，显示了胰岛中存在IAPP mRNA。

相似文献

1
Islet amyloid polypeptide (IAPP) is synthesized in the islets of Langerhans. Detection of IAPP polypeptide and IAPP mRNA by combined in situ hybridization and immunohistochemistry in rat pancreas.胰岛淀粉样多肽（IAPP）在胰岛中合成。通过原位杂交和免疫组化相结合的方法在大鼠胰腺中检测IAPP多肽和IAPP mRNA。
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2
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引用本文的文献

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Am J Pathol. 1993 Jul;143(1):283-91.
2
Islet amyloid polypeptide gene expression in the endocrine pancreas of the rat: a combined in situ hybridization and immunocytochemical study.大鼠内分泌胰腺中胰岛淀粉样多肽基因表达：原位杂交与免疫细胞化学联合研究
Cell Tissue Res. 1993 Dec;274(3):467-74. doi: 10.1007/BF00314543.

本文引用的文献

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A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.一种将DNA限制性内切酶片段放射性标记至高比活度的技术。
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Purification and characterization of a peptide from amyloid-rich pancreases of type 2 diabetic patients.从2型糖尿病患者富含淀粉样蛋白的胰腺中纯化和鉴定一种肽。
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Immunolocalization of islet amyloid polypeptide (IAPP) in pancreatic beta cells by means of peroxidase-antiperoxidase (PAP) and protein A-gold techniques.通过过氧化物酶-抗过氧化物酶（PAP）和蛋白A-金技术对胰岛β细胞中胰岛淀粉样多肽（IAPP）进行免疫定位。
Am J Pathol. 1988 Jan;130(1):1-8.
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Amylin found in amyloid deposits in human type 2 diabetes mellitus may be a hormone that regulates glycogen metabolism in skeletal muscle.在人类2型糖尿病淀粉样沉积物中发现的胰岛淀粉样多肽可能是一种调节骨骼肌糖原代谢的激素。
Proc Natl Acad Sci U S A. 1988 Oct;85(20):7763-6. doi: 10.1073/pnas.85.20.7763.
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Conservation of the sequence of islet amyloid polypeptide in five mammals is consistent with its putative role as an islet hormone.五种哺乳动物中胰岛淀粉样多肽序列的保守性与其作为胰岛激素的假定作用相一致。
Proc Natl Acad Sci U S A. 1989 Aug;86(15):5738-42. doi: 10.1073/pnas.86.15.5738.