Holanda Cecília Maria Carvalho Xavier, Leite Rodrigo Carvalho Holanda, Catanho Maria Teresa Jansem, Souza Grace Maria Lima, Bernardo Filho Mário
Universidade Federal do Rio Grande do Norte (UFRN), Centro de Biociências, Departamento de Microbiologia e Parasitologia, Av. Salgado Filho, no 3000 - 59078-970, Natal/RN, Brazil.
Acta Cir Bras. 2005;20 Suppl 1:126-30. doi: 10.1590/s0102-86502005000700015.
The labeling of red blood cells (C) with 99mTc is employed in clinical nuclear medicine for a variety of diagnostic procedures. Drugs can alter this labeling method and modify the disposition of the radiopharmaceuticals. In this paper, the influence of glucantime on the labeling of blood constituents with 9mTc was reported.
Blood was withdrawn from rats and incubated with glucantime. Stannous chloride and 99mTc were added. After centrifugation, plasma (P) and (C) were isolated. Samples of P and C were precipitated with TCA 5%, centrifuged and insoluble (IF) and soluble fractions (SF) separated. The percentages of total activity injected (%ATI) in C, IF-P and IF-C were calculated (p < 0.05).
The %ATI on C decreased from control to following concentrations of glucantime (6.25%; 12.5%; 25%; 50%; 100%), respectively: 94.06 +/- 1.29 (control) to 77.15 +/- 2.79; to 76.68 +/- 1.88; to 75.15 +/- 2.79; to 72.64 +/- 4.40 and to 63.05 +/- 3.84. On IF-C the %ATI decreased from control to all the concentrations of glucantime (3.125%;6.25%; 12.5%; 25%; 50%; 100%), respectively: 93.34 +/- 1.18 (control) to 78.81 +/- 2.76; to 74.76 +/- 4.82; to 74.02 +/- 5.32; to 64.35 +/- 4.82; to 62.81 +/- 1.97 and to 54.55 +/- 3.58.
This effect was probably due to products present in this drug that may complex with ions (Sn(+2) and 99mTcO4) or have a direct or indirect effect on intracellular stannous ion concentration.
在临床核医学中，用99mTc标记红细胞（C）可用于多种诊断程序。药物会改变这种标记方法并改变放射性药物的处置方式。本文报道了葡甲胺对用99mTc标记血液成分的影响。
从大鼠体内取血，与葡甲胺一起孵育。加入氯化亚锡和99mTc。离心后，分离出血浆（P）和红细胞（C）。用5%三氯乙酸沉淀P和C的样品，离心并分离出不溶物（IF）和可溶部分（SF）。计算C、IF-P和IF-C中注入总活性的百分比（%ATI）（p < 0.05）。
随着葡甲胺浓度（6.25%；12.5%；25%；50%；100%）的增加，C上的%ATI分别从对照值下降：94.06±1.29（对照）降至77.15±2.79；降至76.68±1.88；降至75.15±2.79；降至72.64±4.40；降至63.05±3.84。在IF-C上，%ATI从对照值下降到所有葡甲胺浓度（3.125%；6.25%；12.5%；25%；50%；100%），分别为：93.34±1.18（对照）降至78.81±2.76；降至74.76±4.82；降至74.02±5.32；降至64.35±4.82；降至62.81±1.97；降至54.55±3.58。
这种效应可能是由于该药物中存在的产物，这些产物可能与离子（Sn(+2)和99mTcO4）络合，或者对细胞内亚锡离子浓度有直接或间接影响。
