Holanda C M C X, Silva-Júnior M F, Alves R C, Barbosa V S A, Silva R P, Rocha L G, Medeiros A C
Universidade Federal do Rio Grande do Norte (UFRN), Centro de Biociências, Departamento de Microbiologia e Parasitologia. Av. Salgado Filho, 3000, Natal, RN 59078-970, Brasil.
Cell Mol Biol (Noisy-le-grand). 2009 Nov 15;55(3):23-8.
Radionuclides are used in nuclear medicine by variety of diagnostic procedures. The labeling of red blood cells (RBC) with (99m)Tc is a current method applied in clinical nuclear medicine. Drugs can alter this labeling and modify the disposition of the radiopharmaceuticals. The influence of Rochagan on the labeling of blood constituents with (99m)Tc was reported. Samples of blood were incubated with different concentrations of Rochagan (0%; 6.25%; 12.5%; 25%; 50%; 100%). Stannous chloride and (99m)Tc (3.7MBq/mL) were added. Plasma (P) and (RBC) were isolated and precipitated with thricloroacetic acid 5%. The insoluble (IF) and soluble fractions (SF) were separated. The %ATI in RBC, IF-P and IF-RBC were calculated. The %ATI on RBC decreased significantly (p<0.05) from control to all concentrations of Rochagan, respectively: 90.15 + or - 0.14(control) to 70.80 + or - 4.21; to 64.36 + or - 0.33; to 57.30 + or - 1.56; to 50.28 + or - 2.71; to 42.41 + or - 2.24; on IF-RBC, respectively: 84.70 + or - 0.87(control) to 67.16 + or - 4.38; to 63.63 + or - 2.92; to 59.02 + or - 3.17; to 43.75 + or - 1.00; to 24.15 + or - 0.94 and also on IF-P, respectively: 83.46 + or - 1.09(control) to 50.90 + or - 3.36; to 35.46 + or - 4.13; to 35.78 + or - 2.31; to 28.74 + or - 3.09; to 19.66 + or - 1.34. The analyses were performed by T-Student and Mann Whitney tests, p<0.05. This effect was probably due to products present in Rochagan that may complex with ions or have a direct/indirect effect on intracellular stannous ion concentration.
放射性核素通过多种诊断程序应用于核医学。用（99m）锝标记红细胞（RBC）是目前临床核医学中应用的一种方法。药物可改变这种标记并改变放射性药物的处置。有报道称罗查甘对用（99m）锝标记血液成分有影响。将血液样本与不同浓度的罗查甘（0%；6.25%；12.5%；25%；50%；100%）一起孵育。加入氯化亚锡和（99m）锝（3.7MBq/mL）。分离出血浆（P）和红细胞（RBC），并用5%的三氯乙酸沉淀。分离出不溶部分（IF）和可溶部分（SF）。计算红细胞、IF-P和IF-RBC中的%ATI。从对照组到所有浓度的罗查甘，红细胞上的%ATI均显著降低（p<0.05），分别为：90.15±0.14（对照组）至70.80±4.21；至64.36±0.33；至57.30±1.56；至50.28±2.71；至42.41±2.24；IF-RBC上的%ATI分别为：84.70±0.87（对照组）至67.16±4.38；至63.63±2.92；至59.02±3.17；至43.75±1.00；至24.15±0.94；IF-P上的%ATI分别为：83.46±1.09（对照组）至50.90±3.36；至35.46±4.13；至35.78±2.31；至28.74±3.09；至19.66±1.34。分析采用t检验和曼-惠特尼检验，p<0.05。这种效应可能是由于罗查甘中存在的产物，其可能与离子络合或对细胞内亚锡离子浓度有直接/间接影响。
