Chapman Sean, Oparka Karl J, Roberts Alison G
Programme of Cell-Cell Communication, Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, UK.
Curr Opin Plant Biol. 2005 Dec;8(6):565-73. doi: 10.1016/j.pbi.2005.09.011. Epub 2005 Sep 26.
Engineering of fluorescent proteins continues to produce new tools for in vivo studies. The current selection contains brighter, monomeric, spectral variants that will facilitate multiplex imaging and FRET, and a collection of optical highlighter proteins that might replace photoactivatable-GFP. These new highlighter proteins, which include proteins that have photoswitchable fluorescence characteristics and a protein whose fluorescence can be repeatedly turned on and off, should simplify refined analyses of protein dynamics and kinetics. Fluorescent protein-based systems have also been developed to allow facile detection of protein-protein interactions in planta. In addition, new tags in the form of peptides that bind fluorescent ligands and quantum dots offer the prospect of overcoming some of the limitations of fluorescent proteins such as excessive size and insufficient brightness.
荧光蛋白工程不断为体内研究提供新工具。目前可供选择的荧光蛋白包括更亮的单体光谱变体，有助于多重成像和荧光共振能量转移（FRET），还有一系列光学标记蛋白，有望取代光激活绿色荧光蛋白（PA-GFP）。这些新的标记蛋白，包括具有光开关荧光特性的蛋白以及荧光可反复开启和关闭的蛋白，应能简化对蛋白质动力学和动力学过程的精细分析。基于荧光蛋白的系统也已开发出来，以便在植物中轻松检测蛋白质-蛋白质相互作用。此外，以能结合荧光配体和量子点的肽的形式存在的新标签，为克服荧光蛋白的一些局限性（如尺寸过大和亮度不足）带来了希望。
