Wiedenmann Jörg, Nienhaus G Ulrich
University of Ulm, Department of General Zoology & Endocrinology, 89069 Ulm, Germany.
Expert Rev Proteomics. 2006 Jun;3(3):361-74. doi: 10.1586/14789450.3.3.361.
Fluorescent proteins from the green fluorescent protein family have become indispensable imaging tools for cell biology. A wide variety of these proteins were discovered in nonbioluminescent anthozoa in recent years. Some of them feature exciting new properties, with the possibility to alter their intensity and/or fluorescence color by irradiation with light of specific wavelengths. Fluorescent highlighter proteins enable many interesting applications based on regional optical marking in live cells and tissues. This review provides an overview of photoactivatable marker proteins, with a focus on EosFP, a protein that can be switched from green to red fluorescence by approximately 400-nm light. A variety of applications are presented to illustrate the versatility of EosFP in live-cell imaging.
来自绿色荧光蛋白家族的荧光蛋白已成为细胞生物学中不可或缺的成像工具。近年来，在非生物发光的珊瑚虫中发现了多种此类蛋白。其中一些具有令人兴奋的新特性，有可能通过用特定波长的光照射来改变其强度和/或荧光颜色。荧光标记蛋白使得基于活细胞和组织中的区域光学标记的许多有趣应用成为可能。本综述概述了光激活标记蛋白，重点介绍了EosFP，一种可通过约400纳米的光从绿色荧光切换为红色荧光的蛋白。文中展示了各种应用，以说明EosFP在活细胞成像中的多功能性。
