AcrR第45位氨基酸突变对临床和兽医源大肠杆菌分离株中acrB表达及环丙沙星耐药性的影响
Contribution of mutation at amino acid 45 of AcrR to acrB expression and ciprofloxacin resistance in clinical and veterinary Escherichia coli isolates.
作者信息
Webber Mark A, Talukder Ashraf, Piddock Laura J V
机构信息
Antimicrobial Agents Research Group, Division of Immunity and Infection, The University of Birmingham, Edgbaston, UK.
出版信息
Antimicrob Agents Chemother. 2005 Oct;49(10):4390-2. doi: 10.1128/AAC.49.10.4390-4392.2005.
Abstract
Fluoroquinolone-resistant Escherichia coli isolates which overexpressed acrB and had a substitution at amino acid 45 of AcrR were complemented with wild-type acrR. Complementation led to increased sensitivity to ciprofloxacin and to ethidium bromide, suggesting that mutation at amino acid 45 of AcrR contributes to ciprofloxacin resistance.
摘要
过表达acrB且AcrR第45位氨基酸发生替代的耐氟喹诺酮大肠杆菌分离株用野生型acrR进行了互补。互补导致对环丙沙星和溴化乙锭的敏感性增加，这表明AcrR第45位氨基酸的突变导致了环丙沙星耐药性。
相似文献
1
Contribution of mutation at amino acid 45 of AcrR to acrB expression and ciprofloxacin resistance in clinical and veterinary Escherichia coli isolates.AcrR第45位氨基酸突变对临床和兽医源大肠杆菌分离株中acrB表达及环丙沙星耐药性的影响
Antimicrob Agents Chemother. 2005 Oct;49(10):4390-2. doi: 10.1128/AAC.49.10.4390-4392.2005.
2
Prevalence of antimicrobial resistance and resistance genes in faecal Escherichia coli isolates recovered from healthy pets.从健康宠物粪便中分离出的大肠埃希菌对抗菌药物的耐药性及耐药基因的流行情况。
Vet Microbiol. 2008 Feb 5;127(1-2):97-105. doi: 10.1016/j.vetmic.2007.08.004. Epub 2007 Aug 15.
3
Absence of mutations in marRAB or soxRS in acrB-overexpressing fluoroquinolone-resistant clinical and veterinary isolates of Escherichia coli.在过表达acrB的耐氟喹诺酮类临床和兽医源大肠杆菌分离株中，marRAB或soxRS未发生突变。
Antimicrob Agents Chemother. 2001 May;45(5):1550-2. doi: 10.1128/AAC.45.5.1550-1552.2001.
4
5
Fluoroquinolone resistance and gyrA and parC mutations of Escherichia coli isolated from chicken.从鸡中分离的大肠杆菌的氟喹诺酮耐药性及gyrA和parC基因突变
J Microbiol. 2005 Oct;43(5):391-7.
6
In vivo selection of an Escherichia coli isolate highly resistant to ciprofloxacin and ceftazidime: role of a 4-bp duplication in acrR and ampC overexpression.对环丙沙星和头孢他啶高度耐药的大肠杆菌分离株的体内筛选：acrR基因中4碱基对重复及ampC基因过表达的作用
Int J Antimicrob Agents. 2008 Aug;32(2):196-8. doi: 10.1016/j.ijantimicag.2008.04.001. Epub 2008 Jun 20.
7
Influence of fluoroquinolone consumption in inpatients and outpatients on ciprofloxacin-resistant Escherichia coli in a university hospital.氟喹诺酮类药物在住院患者和门诊患者中的使用对大学医院产 ciprofloxacin 耐药性大肠杆菌的影响。
J Antimicrob Chemother. 2010 Dec;65(12):2650-7. doi: 10.1093/jac/dkq351. Epub 2010 Sep 27.
8
In vivo selection of fluoroquinolone-resistant Escherichia coli isolates expressing plasmid-mediated quinolone resistance and expanded-spectrum beta-lactamase.体内筛选表达质粒介导喹诺酮耐药性和超广谱β-内酰胺酶的耐氟喹诺酮大肠杆菌分离株。
Antimicrob Agents Chemother. 2006 Apr;50(4):1525-7. doi: 10.1128/AAC.50.4.1525-1527.2006.
9
Altered spectrum of multidrug resistance associated with a single point mutation in the Escherichia coli RND-type MDR efflux pump YhiV (MdtF).与大肠杆菌RND型多药耐药外排泵YhiV（MdtF）中的单点突变相关的多药耐药谱改变。
J Antimicrob Chemother. 2007 Jun;59(6):1216-22. doi: 10.1093/jac/dkl426. Epub 2006 Oct 24.
10
Mutant prevention concentration of ciprofloxacin and enrofloxacin against Escherichia coli, Salmonella Typhimurium and Pseudomonas aeruginosa.环丙沙星和恩诺沙星对大肠杆菌、鼠伤寒沙门氏菌和铜绿假单胞菌的突变预防浓度。
Vet Microbiol. 2007 Jan 31;119(2-4):304-10. doi: 10.1016/j.vetmic.2006.08.018. Epub 2006 Aug 17.
引用本文的文献
1
Acquired Bacterial Resistance to Antibiotics and Resistance Genes: From Past to Future.获得性细菌对抗生素的耐药性及耐药基因：从过去到未来
Antibiotics (Basel). 2025 Feb 21;14(3):222. doi: 10.3390/antibiotics14030222.
2
The complex development of psoralen-interstrand crosslink resistance in Escherichia coli requires AcrR inactivation, retention of a marbox sequence, and one of three MarA, SoxS, or Rob global regulators.大肠杆菌中补骨脂素链间交联抗性的复杂发展需要AcrR失活、保留marbox序列以及MarA、SoxS或Rob这三种全局调节因子之一。
Mutat Res. 2025 Jan-Jun;830:111898. doi: 10.1016/j.mrfmmm.2025.111898. Epub 2025 Jan 27.
3
Correlation analysis of whole genome sequencing of a pathogenic Escherichia coli strain of Inner Mongolian origin.内蒙古来源致病性大肠杆菌全基因组测序的关联分析。
Sci Rep. 2024 Jul 5;14(1):15494. doi: 10.1038/s41598-024-64256-5.
4
Antibiotic influx and efflux in Pseudomonas aeruginosa: Regulation and therapeutic implications.铜绿假单胞菌中的抗生素流入和流出：调控与治疗意义。
Microb Biotechnol. 2024 May;17(5):e14487. doi: 10.1111/1751-7915.14487.
5
Quantitative proteomics analysis reveals an important role of the transcriptional regulator UidR in the bacterial biofilm formation of .定量蛋白质组学分析揭示了转录调控因子 UidR 在 细菌生物膜形成中的重要作用。
Front Cell Infect Microbiol. 2024 Mar 22;14:1380747. doi: 10.3389/fcimb.2024.1380747. eCollection 2024.
6
Molecular insights into the determinants of substrate specificity and efflux inhibition of the RND efflux pumps AcrB and AdeB.RND 外排泵 AcrB 和 AdeB 的底物特异性和外排抑制决定因素的分子见解。
Microbiology (Reading). 2024 Feb;170(2). doi: 10.1099/mic.0.001438.
7
Pyridylpiperazine efflux pump inhibitor boosts in vivo antibiotic efficacy against K. pneumoniae.吡啶哌嗪外排泵抑制剂提高了针对肺炎克雷伯菌的体内抗生素疗效。
EMBO Mol Med. 2024 Jan;16(1):93-111. doi: 10.1038/s44321-023-00007-9. Epub 2023 Dec 20.
8
Local Regulator AcrR Regulates Persister Formation by Repression of AcrAB Efflux Pump during Exponential Growth in Aeromonas veronii.在维氏气单胞菌的指数生长期，本地调控因子 AcrR 通过抑制AcrAB 外排泵来调节持续分裂细胞的形成。
Antimicrob Agents Chemother. 2023 Mar 16;67(3):e0096922. doi: 10.1128/aac.00969-22. Epub 2023 Feb 28.
9
Understanding Functional Redundancy and Promiscuity of Multidrug Transporters in under Lipophilic Cation Stress.了解亲脂性阳离子应激下多药转运蛋白的功能冗余和混杂性。
Membranes (Basel). 2022 Dec 14;12(12):1264. doi: 10.3390/membranes12121264.
10
Genomic analysis of the nomenclatural type strain of the nematode-associated entomopathogenic bacterium Providencia vermicola.对与线虫共生的昆虫病原细菌 Providencia vermicola 的命名模式菌株进行基因组分析。
BMC Genomics. 2021 Oct 2;22(1):708. doi: 10.1186/s12864-021-08027-w.
本文引用的文献
1
Role of an acrR mutation in multidrug resistance of in vitro-selected fluoroquinolone-resistant mutants of Salmonella enterica serovar Typhimurium.acrR突变在体外筛选的鼠伤寒沙门氏菌氟喹诺酮耐药突变体多药耐药中的作用
FEMS Microbiol Lett. 2004 Sep 1;238(1):267-72. doi: 10.1016/j.femsle.2004.07.046.
2
Expression of acrB, acrF, acrD, marA, and soxS in Salmonella enterica serovar Typhimurium: role in multiple antibiotic resistance.肠炎沙门氏菌鼠伤寒血清型中acrB、acrF、acrD、marA和soxS的表达：在多重抗生素耐药性中的作用
Antimicrob Agents Chemother. 2004 Apr;48(4):1145-50. doi: 10.1128/AAC.48.4.1145-1150.2004.
3
Role of AcrR and ramA in fluoroquinolone resistance in clinical Klebsiella pneumoniae isolates from Singapore.AcrR和ramA在新加坡临床肺炎克雷伯菌分离株对氟喹诺酮耐药中的作用
Antimicrob Agents Chemother. 2003 Sep;47(9):2831-7. doi: 10.1128/AAC.47.9.2831-2837.2003.
4
Increased expression of the multidrug efflux genes acrAB occurs during slow growth of Escherichia coli.多药外排基因acrAB的表达增加发生在大肠杆菌生长缓慢期间。
FEMS Microbiol Lett. 2002 Jan 22;207(1):91-5. doi: 10.1111/j.1574-6968.2002.tb11034.x.
5
Determination of minimum inhibitory concentrations.最低抑菌浓度的测定
J Antimicrob Chemother. 2001 Jul;48 Suppl 1:5-16. doi: 10.1093/jac/48.suppl_1.5.
6
Absence of mutations in marRAB or soxRS in acrB-overexpressing fluoroquinolone-resistant clinical and veterinary isolates of Escherichia coli.在过表达acrB的耐氟喹诺酮类临床和兽医源大肠杆菌分离株中，marRAB或soxRS未发生突变。
Antimicrob Agents Chemother. 2001 May;45(5):1550-2. doi: 10.1128/AAC.45.5.1550-1552.2001.
7
Genetic characterization of highly fluoroquinolone-resistant clinical Escherichia coli strains from China: role of acrR mutations.中国高氟喹诺酮耐药临床大肠杆菌菌株的遗传特征：acrR突变的作用
Antimicrob Agents Chemother. 2001 May;45(5):1515-21. doi: 10.1128/AAC.45.5.1515-1521.2001.
8
The mar regulon: multiple resistance to antibiotics and other toxic chemicals.mar调控子：对抗生素和其他有毒化学物质的多重耐药性。
Trends Microbiol. 1999 Oct;7(10):410-3. doi: 10.1016/s0966-842x(99)01589-9.
9
The local repressor AcrR plays a modulating role in the regulation of acrAB genes of Escherichia coli by global stress signals.局部阻遏物AcrR在大肠杆菌acrAB基因受全局应激信号调控的过程中发挥调节作用。
Mol Microbiol. 1996 Jan;19(1):101-12. doi: 10.1046/j.1365-2958.1996.357881.x.
10
AcrAB efflux pump plays a major role in the antibiotic resistance phenotype of Escherichia coli multiple-antibiotic-resistance (Mar) mutants.AcrAB外排泵在大肠杆菌多重耐药（Mar）突变体的抗生素耐药表型中起主要作用。
J Bacteriol. 1996 Jan;178(1):306-8. doi: 10.1128/jb.178.1.306-308.1996.
Zotero 插件