Webber M A, Piddock L J
Antimicrobial Agents Research Group, Division of Immunity and Infection, University of Birmingham, Birmingham B15 2TT, United Kingdom.
Antimicrob Agents Chemother. 2001 May;45(5):1550-2. doi: 10.1128/AAC.45.5.1550-1552.2001.
The amount of acrB, marA, and soxS mRNA was determined in 36 fluoroquinolone-resistant E. coli from humans and animals, 27 of which displayed a multiple-resistance phenotype. acrB mRNA was elevated in 11 of 36 strains. A mutation at codon 45 (Arg-->Cys) in acrR was found in 6 of these 11 strains. Ten of the 36 isolates appeared to overexpress soxS, and five appeared to overexpress marA. A number of mutations were found in the marR and soxR repressor genes, correlating with greater amounts of marA and soxS mRNA, respectively.
在36株来自人和动物的耐氟喹诺酮大肠杆菌中测定了acrB、marA和soxS mRNA的量,其中27株表现出多重耐药表型。36株菌株中有11株acrB mRNA水平升高。在这11株菌株中的6株中发现acrR基因第45位密码子(Arg→Cys)发生突变。36株分离株中有10株似乎过表达soxS,5株似乎过表达marA。在marR和soxR阻遏基因中发现了一些突变,分别与marA和soxS mRNA的量增加相关。