Ragupathi G, Prabhasankar P, Sekharan P C, Annapoorani K S, Damodaran C
R&D Division, Forensic Sciences Department, Madras, India.
J Chromatogr. 1992 Feb 14;574(2):267-71.
A method is reported for the solid-state fluorodensitometric determination of haptens in hapten-protein conjugates. The applicability of the method is shown using the bovine serum albumin conjugate of cleistanthin B, a toxic glycoside of the plant Cleistanthus collinus. This non-destructive technique, which is relatively simple, sensitive, rapid and versatile, can be used for the determination of protein conjugates of fluorogenic haptens.
报道了一种用于固态荧光密度法测定半抗原-蛋白质缀合物中半抗原的方法。使用植物铁海棠的有毒糖苷克利斯坦丁B的牛血清白蛋白缀合物展示了该方法的适用性。这种相对简单、灵敏、快速且通用的非破坏性技术可用于测定荧光半抗原的蛋白质缀合物。
