Annapoorani K S, Periakali P, Ilangovan S, Damodaran C, Sekharan P C
J Anal Toxicol. 1984 Jul-Aug;8(4):182-6. doi: 10.1093/jat/8.4.182.
Spectrofluorometric quantitation of a lignan lactone, diphyllin, and its glycosides cleistanthin A and cleistanthin B, the active principles of the poisonous plant Cleistanthus collinus, is reported for the first time. Thin layer chromatographic resolution of the components precedes the fluorometric measurements. The method is highly reproducible and sensitive to 0.1 microgram/mL for diphyllin and to 1 microgram/mL for cleistanthins A and B in ethanol. The technique is employed for the assay of several biospecimens of forensic and clinical importance.
首次报道了用荧光分光光度法对木脂素内酯、二叶豆素及其糖苷（克利斯坦辛A和克利斯坦辛B）进行定量分析，这些物质是有毒植物二叶豆的活性成分。在荧光测量之前，先对各成分进行薄层色谱分离。该方法具有高度的重现性，对乙醇中二叶豆素的检测灵敏度为0.1微克/毫升，对克利斯坦辛A和B的检测灵敏度为1微克/毫升。该技术用于分析一些具有法医和临床重要性的生物标本。
