Ragupathi G, Prabhasankar P, Sekharan P C, Annapoorani K S, Damodaran C
R & D Division, Forensic Sciences Department, Madras, India.
J Immunoassay. 1992;13(3):321-38. doi: 10.1080/15321819208021236.
Enzyme-linked immunosorbent assay is reported for the estimation of cleistanthin A, a major constituent of the toxic plant Cleistanthus collinus. Rabbit antibodies were obtained by immunisation with cleistanthin A hemisuccinate-BSA conjugate and the ELISA developed thereupon could detect cleistanthin A at as low a concentration as 3 ng/ml. Cross-reactivity studies with structural analogs as well as with other phytotoxins and drugs of common occurrence established the suitability of the ELISA to specifically monitor the C. collinus marker molecules in emergency clinical and forensic cases. The simplicity and specificity make the ELISA superior to the other available techniques.
据报道，酶联免疫吸附测定法用于估算有毒植物鸡骨常山（Cleistanthus collinus）的主要成分鸡骨常山碱A。通过用鸡骨常山碱A半琥珀酸酯 - 牛血清白蛋白共轭物免疫获得兔抗体，据此开发的酶联免疫吸附测定法能够检测低至3 ng/ml浓度的鸡骨常山碱A。与结构类似物以及其他常见植物毒素和药物的交叉反应研究证实了该酶联免疫吸附测定法适用于在紧急临床和法医案件中特异性监测鸡骨常山的标记分子。该酶联免疫吸附测定法的简单性和特异性使其优于其他现有技术。
