Houzé P, de Reynies A, Baud F J, Benatar M F, Pays M
Département de Biochimie-Pharmacologie-Toxicologie, C.H.R. de Versailles, Le Chesnay, France.
J Chromatogr. 1992 Feb 14;574(2):305-12. doi: 10.1016/0378-4347(92)80044-q.
A method was developed for the separation and measurement of chloroquine and three metabolites (desethylchloroquine, bisdesethylchloroquine and 4-amino-7-chloroquinoline) in biological samples by ion-pair high-performance liquid chromatography with UV detection. The method uses 2,3-diaminoaphthalene as an internal standard and provides a limit of detection between 1 and 2 ng/ml for chloroquine and its metabolites. The assay was linear in the range 12.5-250 ng/ml and the analytical recovery and reproducibility were sufficient. The assay was applied to the analysis of biological samples from a patient undergoing chloroquine chemoprophylaxis and a patient who had ingested chloroquine in a suicide attempt.
