尿液中氯喹及其代谢物的测定：一种基于离子对萃取的现场方法。
Determination of chloroquine and its metabolites in urine: a field method based on ion-pair extraction.
作者信息
Bergqvist Y, Hed C, Funding L, Suther A
出版信息
Bull World Health Organ. 1985;63(5):893-8.
A new straightforward photometric method for the assay of the antimalarial drug chloroquine and its metabolites in urine is described. The method involves an ion-pair extraction procedure with dichloromethane using the acid-base indicator bromthymol blue as counter-ion. The ion pair formed with chloroquine in the organic phase is yellow, and absorbance is measured at lambda = 410 nm using a filter photometer. The absorbance is a linear function of concentration up to 400 mumol/l (120 mg/l) chloroquine. The method is suitable for the determination of chloroquine and its metabolites in urine down to a limiting concentration of about 10 mumol/l (3 mg/l). Additionally, the method is suitable for semiquantitative visual estimation of the concentration of chloroquine in urine. A single dose of 5 mg/kg chloroquine base could be determined in urine from two volunteers for at least 8 days after administration of the drug. The results obtained for the analysis of chloroquine and its metabolites with the colorimetric method described here correlate well with those obtained using high performance liquid chromatography.
本文描述了一种用于测定尿液中抗疟药物氯喹及其代谢产物的新型直接光度法。该方法涉及使用酸碱指示剂溴百里酚蓝作为抗衡离子，用二氯甲烷进行离子对萃取过程。在有机相中与氯喹形成的离子对为黄色，使用滤光光度计在λ = 410 nm处测量吸光度。吸光度在氯喹浓度高达400 μmol/l（120 mg/l）时是浓度的线性函数。该方法适用于测定尿液中氯喹及其代谢产物，最低限度浓度约为10 μmol/l（3 mg/l）。此外，该方法适用于尿液中氯喹浓度的半定量目视估计。在两名志愿者服用5 mg/kg氯喹碱单剂量药物后，至少8天内可在尿液中检测到该药物。本文所述比色法分析氯喹及其代谢产物的结果与高效液相色谱法所得结果相关性良好。
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