Howard Wendy A, Bible Jon M, Finlay-Dijsselbloem Eva, Openshaw Sam, Dunn-Walters Deborah K
Department of Immunobiology, Guy's King's and St Thomas' Medical School, Guy's Campus, London, SE1 9RT, UK.
Immunogenetics. 2005 Oct;57(9):655-64. doi: 10.1007/s00251-005-0020-2. Epub 2005 Oct 18.
The combined processes of immunoglobulin (IG) gene rearrangement and somatic hypermutation allow for the creation of an extremely diverse antibody repertoire. Knowledge of the germline sequence of the IG genes is required so that hypermutation and the affinity matured humoral response can be properly studied. Variable region genes can be arranged into subgroups; in humans, there are 11 IGLV subgroups and 6 IGKV subgroups. The rhesus macaque (Macaca mulatta) is a relevant non-human primate model for human immunological systems. A number of macaque IGHV, IGHD and IGHJ genes have already been reported. We have also previously reported a number of macaque IGKV genes. Here we report the isolation of new macaque IGLV genes by polymerase chain reaction amplification from macaque genomic DNA using primers based on the human sequences. Nine IGLV1, 10 IGLV2, 21 IGLV3, 5 IGLV4 and 7 IGLV5 germline genes for the macaque were found, the open-reading frames of which exhibit high homology to their human counterparts (>89.3, >88.6, >89.0, >94.7 and >87.1%, respectively).
免疫球蛋白（IG）基因重排和体细胞超突变的联合过程使得能够产生极其多样的抗体库。需要了解IG基因的种系序列，以便能够正确研究超突变和亲和力成熟的体液免疫反应。可变区基因可分为不同的亚组；在人类中，有11个IGLV亚组和6个IGKV亚组。恒河猴（猕猴）是研究人类免疫系统的一种相关非人灵长类动物模型。已经报道了一些猕猴的IGHV、IGHD和IGHJ基因。我们之前也报道过一些猕猴的IGKV基因。在此，我们报告通过聚合酶链反应扩增，从猕猴基因组DNA中使用基于人类序列的引物分离出新的猕猴IGLV基因。发现了9个猕猴的IGLV1、10个IGLV2、21个IGLV3、5个IGLV4和7个IGLV5种系基因，其开放阅读框与人类对应基因具有高度同源性（分别>89.3%、>88.6%、>89.0%、>94.7%和>87.1%）。
