Neubecker T A, Miller N S, Asquith T N, Driscoll K E
Miami Valley Laboratories, Procter and Gamble Company, Cincinnati, OH 45239-8707.
J Chromatogr. 1992 Feb 14;574(2):340-3. doi: 10.1016/0378-4347(92)80049-v.
To better define involvement of mast cell derived mediators in the pulmonary response to fibrogenic dusts, a rapid and accurate method was required to analyze samples of bronchoalveolar lavage fluid for histamine. Samples of rat lung lavage were analyzed for histamine via high-performance cation-exchange chromatography coupled with post-column derivatization with o-phthaldialdehyde. The fluorescent derivative could be detected to ca. 1 ng/ml of lavage. Recoveries averaged 94.2% with an average relative standard deviation of +/- 5.3%. There were no correlations between amount or fibrogenicity of inhaled dust and subsequent release of histamine into lavage fluid.
为了更好地确定肥大细胞衍生介质在肺部对致纤维化粉尘反应中的作用，需要一种快速准确的方法来分析支气管肺泡灌洗液中的组胺样本。通过高效阳离子交换色谱结合邻苯二甲醛柱后衍生化法分析大鼠肺灌洗样本中的组胺。荧光衍生物的检测限可达约1 ng/ml灌洗液。回收率平均为94.2%，平均相对标准偏差为±5.3%。吸入粉尘的量或致纤维化能力与随后组胺释放到灌洗液中的量之间没有相关性。
